BID蛋白促细胞凋亡增殖失衡作用与IgA肾病患者病情的关系

Promotion of disequilibrium of cell proliferation and apoptosis of BID as a potential mechanism of IgAN

目的探讨IgA肾病(IgAN)不同病变程度肾组织中BID蛋白的表达与细胞凋亡、增殖的关系。方法免疫组化法检测肾组织PCNA、BID蛋白和FN,用原位末端标记法(TUNEL)结合光镜形态学检测细胞凋亡情况。结果随IgAN病变程度加重,BID蛋白、PCNA、凋亡细胞及FN表达逐渐增强(P<0.05),Ⅳ级最强(PCNA:Ⅲ、Ⅳ相当);肾小球内BID蛋白与凋亡细胞比值、PCNA、FN表达,肾小球硬化率及尿蛋白排泄量正相关,与eGFR负相关(P<0.01)。结论BID蛋白可通过促进细胞凋亡增殖失平衡促进IgAN病程进展,可能为判断预后的一项重要病理指标。

Objective To explore the expression of BID protein and the relation ship of cell proliferation with apoptosis at different stages of IgAN. Methods The expression of BID protein,PCNA and FN in renal tissue was detected by immunohistochemistry, TUNEL and light microscopy were used to detect apoptotic cells. Results With the progress of IgAN, the expression of BID protein,PCNA the number of apoptotic cells and FN increased gradually （ P 〈 0. 05 ）, especially in Ⅳ group （ PCNA ： Ⅳ is in the same rank as Ⅲ group）, The expression of BID protein in glomcrulus positively correlated with the rate of apoptotic cells,the express of PCNA and FN,the rate of glomerular sclerosis and urine protein, and negatively correlated with glomerular filtration rate （ P 〈 0. 01 ）. Conclusion BID protein accelerates the progression of IgAN through promoting disequilibrium of cell proliferation and apoptosis, so it may function as a marker for prognosis.