摘要
目的探讨自体骨髓单个核细胞(BM-MNC)移植用于大鼠缺血后肢的治疗后实现血管再生的能力。方法建立大鼠后肢缺血动物模型,将取于自体的BM-MNC制成细胞悬液注射于缺血部位,分别在移植后2和30 d时行动脉造影,用免疫组化方法检测内皮祖细胞(EPC),毛细血管密度以及测定血管内皮生长因子(VEGF)的表达。结果缺血肌组织中的EPC含量增高(P<0.01)。BM-MNC移植组在移植早期VEGF表达显著增高(P<0.01)。细胞移植后30 d BM-MNC移植组毛细血管密度明显高于其他组(P<0.01),血管造影可见侧支循环建立。结论自体骨髓单个核细胞移植于大鼠后肢缺血区能促进血管新生,改善侧支循环,可望成为一种简单有效的治疗下肢缺血的方法。
Objective Using hone marrow mononuclear cells （BM-MNCs） transplantation to induce vaseulogenesis in ischemic hindlimb, for development of an easier and safer therapy for ischemic arterial disease at the lower extremity. Methods BM-MNCs isolated from rat bone marrow were transplanted into ischemie area of rat. The animals were killed at day 2 and day 30 after taking the angiography. Differentiation of the endothelial progenitor cells （EPCs）, capillaries density and vascular endothelial growth factor （VEGF） expression of the local tissue was analyzed by immunohistochemical staining. Results Increased EPCs were found in muscles in ischemie muscle （P 〈0. 01 ）. The density of capillaries was much higher in BM-MNC implantation group after 30 days （P 〈0. 01 ）. In angiography, collateral arteries were significantly increased in ischemic hindlimbs （P 〈 0. 01 ）. The expression of VEGF was significantly higher in the transplanted muscles after 2 days （P 〈0. 01 ）. Conclusion BM-MNCs can induce vasculogenesis in the ischemie muscle. Implantation of autologous BM-MNCs is a simple and efficient therapy for ischemic hindlimbs.
出处
《基础医学与临床》
CSCD
北大核心
2009年第2期183-187,共5页
Basic and Clinical Medicine
