小鼠胃白念珠菌感染动物模型的建立

Establishment of a mouse model of stomach infection with candida albicans

目的:通过降低小鼠免疫力并给小鼠胃肠道造成溃疡,建立小鼠胃白念珠菌感染动物模型.方法:昆明小鼠110只随机分成3组.对照组Ⅰ给予浓度5.5×1012白念珠菌菌悬液0.5mL灌胃;对照组Ⅱ腹腔内注射浓度40g/L的环磷酰胺溶液0.02mL/g体质量,并用3g/L冰醋酸溶液0.5mL灌胃;模型组用对照Ⅱ组方法灌胃,2h后给予浓度5.5×1012白念珠菌菌悬液0.5mL灌胃.于第10天取小鼠胃组织行真菌镜检、组织病理检查,同时取所有真菌镜检阳性胃组织经念珠菌显色培养基培养,观察菌落.结果:模型组小鼠胃组织内发现念珠菌孢子、芽生孢子、假菌丝及大量成团菌丝.模型组与对照组Ⅰ真菌镜检及组织病理HE染色阳性率比较有显著性差异(χ2=40.763,40.526,均P<0.01),与对照组Ⅱ比较也有显著性差异(χ2=58.964,44.074,均P<0.01).结论:通过降低小鼠免疫力并造成胃溃疡,应用白念珠菌感染可以建立小鼠胃白念珠菌感染动物模型.

AIM： To establish a mouse model of stomach with candida albicans infection on hypoimmunity and ulceric mouse. METHODS： Kunming mice were randomly divided into 3 groups. Mice in control group were given 0.5 mL 5.5 × 10^12 candida albicans suspension by gastric intubation; mice in control group Ⅱ were given cyclophosphamide （40 g/L） through intraperitoneal injection by 0.02 mL per gram （weight） and lavaged with 0.5 mL 3 g/L glacial acetic acid; mice in model group were first lavaged in the same way as control group Ⅱ, then 2 hours later, were given intragastric administration with 0.5 mL 5.5 × 10^12 candida albicans suspension. On day 10, microscopic and histopathological examination were performed on stomach tissue of mice. Positive fungi under microscopy were all cultivated with CHROMagar, and observed. RESULTS： Monilia sporus, blastospore, pseudohypha and a bulk of hypha in stomach were observed in model group. The differences in positive rates upon microscopic and histopathological examination between model group and control group Ⅰ were significant （χ^2 = 40.763, 40.526, both P 〈 0.01）. There was also significant difference in positive rate between model group and control group Ⅱ（χ^2 = 58.964, 44.074, both P 〈 0.01）. CONCLUSION： By inhibiting immunity and inducing peptic ulcer, we can establish a mouse model of stomach with candida albicans infection.