摘要
目的:通过降低小鼠免疫力并给小鼠胃肠道造成溃疡,建立小鼠胃白念珠菌感染动物模型.方法:昆明小鼠110只随机分成3组.对照组Ⅰ给予浓度5.5×1012白念珠菌菌悬液0.5mL灌胃;对照组Ⅱ腹腔内注射浓度40g/L的环磷酰胺溶液0.02mL/g体质量,并用3g/L冰醋酸溶液0.5mL灌胃;模型组用对照Ⅱ组方法灌胃,2h后给予浓度5.5×1012白念珠菌菌悬液0.5mL灌胃.于第10天取小鼠胃组织行真菌镜检、组织病理检查,同时取所有真菌镜检阳性胃组织经念珠菌显色培养基培养,观察菌落.结果:模型组小鼠胃组织内发现念珠菌孢子、芽生孢子、假菌丝及大量成团菌丝.模型组与对照组Ⅰ真菌镜检及组织病理HE染色阳性率比较有显著性差异(χ2=40.763,40.526,均P<0.01),与对照组Ⅱ比较也有显著性差异(χ2=58.964,44.074,均P<0.01).结论:通过降低小鼠免疫力并造成胃溃疡,应用白念珠菌感染可以建立小鼠胃白念珠菌感染动物模型.
AIM: To establish a mouse model of stomach with candida albicans infection on hypoimmunity and ulceric mouse.
METHODS: Kunming mice were randomly divided into 3 groups. Mice in control group were given 0.5 mL 5.5 × 10^12 candida albicans suspension by gastric intubation; mice in control group Ⅱ were given cyclophosphamide (40 g/L) through intraperitoneal injection by 0.02 mL per gram (weight) and lavaged with 0.5 mL 3 g/L glacial acetic acid; mice in model group were first lavaged in the same way as control group Ⅱ, then 2 hours later, were given intragastric administration with 0.5 mL 5.5 × 10^12 candida albicans suspension. On day 10, microscopic and histopathological examination were performed on stomach tissue of mice. Positive fungi under microscopy were all cultivated with CHROMagar, and observed.
RESULTS: Monilia sporus, blastospore, pseudohypha and a bulk of hypha in stomach were observed in model group. The differences in positive rates upon microscopic and histopathological examination between model group and control group Ⅰ were significant (χ^2 = 40.763, 40.526, both P 〈 0.01). There was also significant difference in positive rate between model group and control group Ⅱ(χ^2 = 58.964, 44.074, both P 〈 0.01).
CONCLUSION: By inhibiting immunity and inducing peptic ulcer, we can establish a mouse model of stomach with candida albicans infection.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第3期303-306,共4页
World Chinese Journal of Digestology
关键词
白念珠菌
胃
溃疡
小鼠
动物模型
Candida albicans
Stomach
Ulcer
Mice
Animal model