脐血单个核细胞IL-12和IFN-γ表达的观察

Observation the Expression of IL-12,IFN-γand Their mRNA in Cord Blood Mononuclear Cells

本文探讨新生儿脐血单个核细胞（CBMC）白细胞介素12（IL－12）和γ－干扰素（IFN－γ）基因表达水平；观察重组IL－12（rIL－12）对CBMCIFN－γ基因表达的影响。采用逆转录－聚合酶链式反应（RT－PCR）测定8例新生儿CBMCIL－12p_(40)mRNA和IFN－ymRNA表达，用ELISA法测新生儿CBMC细胞培养上清液中IFN－γ浓度。结果显示：新生儿CBMC在体外受刺激后表达的IL－12p_(40)mRNA、IFN－γmRNA均较成人PBMC低下；新生儿CBMC培养上清液中IFN－γ水平明显低于成人，rIL－12能增强PMA和抗CD_3单克隆抗体（McAb）刺激的新生儿CBMC产生IFN－γ，使其达到成人水平。提示：缺乏IL－12调节作用可能是新生儿CBMCIFN－γ基因表达水平不足的重要原因之一。

The objective of this study was to explore the levels of interleukin 12(IL-12) and IFN-γ gene expression in neonatal cord blood mononuclear cells (CBMC). The expression of IL-12 p40 mRNA and IFN-γ mRNA in neonatal CBMC of 8 cases were determined with reverse transcription-polymerase chain reaction (RT-PCR),and the concentration of IFN-γ in supernatant of cultured neonatal CBMC was determined with enzyme linked immunosorbent assay (ELISA). The results showed that the expression of IL-12p40 mRNA and IFN-γ mRNA in CBMC were lower than those of adults PBMC after being stimulated in vitro,the level of IFN-γ in supernatant of cultured neonatal CBMC was significantly lower than that of adults, rIL-12 could enhance IFN-γ producing in CBMC stimulated by PMA and anti-CD3 monoclonal antibody up to the level of adults. The results of this study suggest that lack of regulation by IL-12 may be an important cause of insufficient IFN-γ gene expression in neonatal CBMC.