逆转录-聚合酶链反应银染分析IRF-1基因在白血病中的表达

Study on expression of IRF1 gene in human leukemia with RTPCR and the silver sequence techniques.

目的：初步分析白血病细胞中ＩＲＦ－１基因表达水平的变化。方法：采用逆转录－聚合酶链反应，银染分析检测２４例白血病患者和３种白血病细胞系中ＩＲＦ－１基因表达水平。结果：３例白血病患者未检测到ＩＲＦ－１基因表达，５例患者及ＨＬ－６０、ＨＩＭｅｇ细胞系ＩＲＦ－１基因表达明显降低。结论：部分白血病患者的发病机制可能与ＩＲＦ－１基因表达缺失或下降有关；ＩＲＦ－１基因表达水平的变化可能对部分白血病患者的疗效观察及预后有参考价值。

Objective:To explore preliminarily the changes of IRF1 gene expression in leukemias.Methods:The expression of IRF1 gene in 24 leukemia patients and 3 leukemic cell lines were studied with RTPCR and the silver sequence techniques.Results:No expression of IRF1 gene in 3 patients,and significantly decreased expressions in 5 patients and in HL60 and HIMeg cell lines were revealed.Conclusion: The mechanism of human leukemogenesis may be partly associated with no or lowexpression of IRF1 gene.