恶性淋巴瘤患者血清中IL-2、sIL-2R、IL-6、IL-8和TNFα的测定

Measurement of five cytokines in the serum of malignant lymphoma patients

目的：探讨血清中细胞因子变化在恶性淋巴瘤（ＭＬ）中的意义。方法：采用放射免疫分析法及酶联免疫吸附法测定了４９例ＭＬ患者血清中白细胞介素２（ＩＬ－２）等５种细胞因子浓度。结果：除肿瘤坏死因子α（ＴＮＦα）外，大部分初治、复发患者中治疗前ＩＬ－２，可溶性白细胞介素２受体（ｓＩＬ－２Ｒ），ＩＬ－６，ＩＬ－８升高；治疗有反应的下降，完全缓解时与对照组无差异；治疗无反应的仍保持高水平。ＩＬ－２及ｓＩＬ－２Ｒ升高与患者的临床分期有关，Ⅲ、Ⅳ期患者较Ⅰ、Ⅱ期明显增高；ｓＩＬ－２Ｒ与肿瘤负荷有关；ＩＬ－６与患者有无Ｂ组症状相关，Ｂ组患者明显高于Ａ组，而与分期无关；ＩＬ－８升高与临床分期及Ｂ组症状有关，与其他临床血液学指标无关。结论：细胞因子的变化可以作为观察ＭＬ病情及监测治疗反应的一种手段。

Objective:To study the clinical significance of serum cytokine levels in patients with malignant lymphoma(ML).Methods:Serum levels of five cytokines and receptor were measured in 49 patients with ML by RIA and ELISA.Results:Except TNFα,significantly higher pretreatment levels of interleukin2(IL2),soluble interleukin2 receptor(sIL2R), interleukin6(IL6) and interleukin8(IL8) were observed in most of ML patients at diagnosis or relapse as compared with controls(P<0.05). Cytokine levels declined in responding patients after therapy and there were no differences between CR cases and controls,the cytokine levels remained elevated in nonresponding patients. The levels of IL2 and sIL2R correlated with the clinical stage , which were significantly higher in patients in stage Ⅲ、Ⅳ.The increase of sIL2R correlated with the tumor burden.The level of IL6 was higher in patients presenting B symptoms and had no correlation with the clinical stage.The elevated IL8 level correlated with the clinical stage and presenting B symptoms of ML patients and had no correlation with other clinicalhematological parameters.Conclusion:The changes of cytokines may be served as a means to observe the condition of ML patients and supervise their response to treatment.