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DAPT抑制人胶质瘤细胞增殖的体内外实验研究 被引量：1

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摘要 目的研究Notch信号通路抑制剂DAPT对人胶质瘤细胞增殖的影响。方法应用不同剂量DAPT处理胶质瘤细胞系U251,噻唑蓝(MTT)比色试验检测细胞增殖,流式细胞仪检测细胞周期,建立人胶质瘤裸鼠模型,观察肿瘤生长情况。结果DAPT呈剂量依赖性抑制U251细胞增殖,在浓度为0.25μmol/L时即有抑制作用,浓度为5.00μmol/L抑制效果更明显;随DAPT浓度增大G0/G1期细胞比率明显增多(P<0.01),而S期细胞比率明显减少(P<0.01),使细胞周期阻滞于G0/G1期;用药6周后DAPT处理组裸鼠皮下肿瘤体积明显缩小,平均瘤重对照组为(2.23±0.54)g,DAPT组为(0.97±0.29)g(P<0.01),抑瘤率为56.50%。结论DAPT通过阻断Notch信号通路,在体内外有效地抑制人胶质瘤细胞的增殖,提示抑制Notch信号通路的活性可能对胶质瘤的治疗起到一定的作用。
作者 杨永凯 杨卫忠 石松生 王春华 陈春美 李军涛
机构地区 福建医科大学附属协和医院神经外科
出处 《福建医药杂志》 CAS 2009年第1期83-85,共3页 Fujian Medical Journal
关键词 胶质瘤 DAPT 信号通路 增殖
分类号 R739.41 [医药卫生—肿瘤]
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参考文献8

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二级参考文献9

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福建医药杂志
2009年 第1期

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