肝硬化时左右半肝硬化程度对比研究

The investigation on the morphous of left and right half-liver cirrhosis

目的研究乙型肝炎肝硬化病人左右半肝形态及肝硬化病人肝组织标本的HE染色和肝硬化相关因子基质金属蛋白酶-2和碱性成纤维细胞生长因子-2的表达。方法对30例乙肝肝硬化致门脉高压征行脾切除术和乙肝肝硬化失代偿行肝移植手术病人行CT检查,手术中标本摄片,留取形态学资料。同时留取此30例病人肝组织标本,进行HE染色,根据肝硬化标准判断肝纤维化程度并行免疫组化染色检测MMP2和bFGF2的表达。结果1)发现右半肝萎缩较左半肝萎缩明显增多;2)HE染色亦显示右半肝肝硬化较左半肝肝硬化明显增多;3)MMP2在病例组左半肝的表达明显高于对照组左半肝表达,有统计学差异;4)bFGF2在病例组左半肝表达与对照组左半肝表达经检验无统计学差异。在病例组右半肝表达明显高于对照组右半肝表达,有统计学差异。结论肝硬化时左右半肝纤维化程度存在明显地差异性,为我们今后治疗肝硬化合并肝癌,手术切除肝组织大小及部位提供了新的理论依据。

Objective To investigate the morphous of left and right half- liver cirrhosis and the expression of related factor of hepatic cirrhosis matrix metalloproteinase 2 （ MMP - 2 ） and basic fibroblast growth factor 2 （ bFGF - 2 ） . Methods 30 specimens of patients with cirrhotic portal hypertension caused by infection of hepatitis B virus （HBV） cirrhosis and liver transplantation for decompensation stage of posthcpatitic cirrhosis had CT scan, the samples were observed with morphologic examination and HE staining method according to cirrhosis stage. Immunohistoebemistry was used to examine the expression of MMP2 and bFGF2 in the tissues. Results In the 30 eases with liver cirrhosis, Comparison of morphological changes, the right lobe of liver had a more significant sign of atrophy compared with the left lobe of liver. HE staining showed the degree of cirrhosis in the right lobe of liver was more than that he left lobe. The expression of MMP2 and bFGF2 in the patients group was more obvious than that in the control group, and there was a significant difference between them. Conclusion The difference between the degree of left and right half- liver hepatic fibrosis in hepatic cirrhosis was significant. There is a close relationship between MMP2, bFGF2 and left and right half- liver fibrosis in hepatic cirrhosis. This research provides some new theoretical basis for the location and the size of the surgical excision of hepatocareinoma with cirrhosis.