醛固酮合酶基因多态性与原发性高血压相关性研究

Study on the relation between the CYP11B2 gene polymorphisms and essential hypertension

目的探讨醛固酮合酶基因(CYP11B2)-344T/C多态性与原发性高血压的相关性。方法采用关联分析,收集湖南地区汉族男性原发性高血压患者100例,正常对照100名。应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)对2组对象的CYP11B2-344T/C多态进行分析。结果2组对象CYP11B2基因型(TT型、CT型和CC型)的频率差异无统计学意义(χ2=0.34,P>0.05),等位基因的频率差异也无统计学意义(χ2=0.28,P>0.05),但各组内等位基因T的频率(原发性高血压组:67.67%)高于等位基因C(32.33%);对照组T69.67%高于C 30.33%。结论多基因联合分析显示,在男性患者中,CYP11B2-344T/C多态性与原发性高血压无明显相关,但CYP11B2-344各组内等位基因T的频率高于等位基因C。

Objective To explore the relation between the polymorphism-344T/C of aldosterone synthase gene （CYP11B2） and essential hypertension. Methods Association analysis was employed and the single nucleotide polymorphisms of genomic DNA from 100 patients with essential hypertension and 100 normal Han subjects in Hunan randomly selected according to a standard were genotyped by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis. Results There was no significant difference of gene frequency （TT, TC, and CC ） between the hypertensive and the control groups （X^2 = 0.34, P 〉 0.05 ） , so did the distributions of T and C alleles in both groups （X^2=0. 28, P 〉 0.05 ）. The frequency of T allele was higher than that of C allele in both groups （ hypertension： 67.67% vs32.33%, P〈0.05; controls： 69.67% vs 30.33%, P〈0.05）. Conclusions Polygene associated assay shows that the polymorphism of CYP11B2 -344C has not obvious connection with essential hypertension in male case, while the frequency of T allele of CYP11 B2 -344C in both groups was higher than that of C allele, which has obvious connection with essential hypertension.