化痰散结中药对甲状腺细胞增殖与凋亡影响的实验研究

Experimental Study on the Effect of Removing Phlegm and Resovling Mass Medicinal Herbs on Proliferation and Apoptosis of FRTL Cells

目的:研究化痰散结中药甲肿消对甲状腺FRTL细胞增殖与凋亡的影响。方法:用水煮浸渍提取方法制备甲肿消提取液,甲状腺FRTL细胞株采用含6H的F12培养基培养。中药提取液作用培养细胞,以MTT法测定不同浓度甲肿消对FRTL细胞的增殖影响;采用Annexin V/PI双染法,应用流式细胞仪检测细胞凋亡;PI单染,流式DNA定量法检测细胞周期。结果:甲肿消作用24 h,细胞半数抑制浓度IC50=0.8 mg/ml(含生药浓度),最大抑制率浓度为4.0 mg/ml。甲肿消作用后G0/G1期细胞增多,S和G2/M期细胞减少,增值指数(PI)显著降低,细胞凋亡增加;同时发现甲肿消对细胞增殖与凋亡的影响与作用剂量、作用时间成正相关。结论:甲肿消通过阻滞细胞从G0/G1期向S期转换,从而抑制细胞增殖,同时可诱导FRTL细胞的凋亡,并与作用剂量和作用时间正相关。

Objective： To investigate the effect of Chinese Herb JiaZhongXiao （JZX） on proliferation and apoptosis of FRTL cells. Methods JZX was prepared by tradition method that cooked in distilled water. FRTL cells was grown in F12 medium with 6H. JZX was added to the medium. The effect of the medicine on proliferation of FRTL cells was used an MTT- based assay. The apoptosis index was checked by flow eytometer with Anexin V/PI. Cell cycle was analyzed by flow eytometer too, Results FRTL cells was treated with JZX for 24 h, the IC50 was 0.8 mg/ml, and maximal inhibition occurred at the concentration of 4 mg/ml. The number of cells in G0/G1 phase was increased obviously, but the cells in S and G2/M phase and the proliferation index （PI） was decreased after treated by JZX. The apoptosis rate was increased too. The effect of JZX on inhibiting proliferation and inducing apoptosis was related to the dosages and the treated time. Conclusions： JZX inhibits cell proliferation through delaying the G0/G1 phase in FRTL ceils, and induces apoptosis remarkably which related to the dosages and the treated time.