摘要
从烟台昆嵛山分离到一株野生灵芝Gl-01,根据rDNAITS序列的系统发育分析,将其鉴定为赤芝(Ganodermalucidum(Fr.)Karst)。通过对这株野生灵芝在液体培养基中的耐硒能力测定以及不同培养时间的富硒特性考察,表明该野生灵芝菌株具有较强的富硒能力,其菌丝硒含量达到5.14mg/g,富硒率达到4.05%,分别为对照菌株SK5的2.4倍和1.5倍。
One wild Ganoderma strain Gl-01 was isolated from Kunyu mountain in Yantai, Shandong province, China, and was identified as a known species of Ganodermalucidum （Fr） Karstbasedon the sequenceanalysis of rDNA ITS, Itsabilityofselenium （Se）-resistant and characteristics of selenium accumulation with different culture time were investigated by shake flask in Se-containing medium. The results indicated that the wild strain showed highly Se-enrichment abilities, and Se content ofmycelia and Se-enrichment rate were up to 5.14 mg/g and 4.05%, which were 2.4 folds and 1-5 folds ofthe control strain Sk5, respectively. The results provided a reference on developing new Se-enrichment strains from the wild Ganoderma lucidum.
出处
《中国酿造》
CAS
北大核心
2009年第3期47-49,83,共4页
China Brewing
基金
山东省教育厅科技计划项目资助(J06N02)
鲁东大学博士基金资助(LY20063301)
关键词
灵芝
分子鉴定
富硒
Ganoderma lucidum
molecular identification
Se accumulation
