利用木糖产油酵母的微波诱变选育

Screening of oleaginous yeast by utilizing xylose by microwave irradiation mutation

采用微波处理方式,对1株利用木糖产油酵母菌圆红冬孢酵母菌HYT-05进行诱变处理。经过目检、初筛和复筛,最终得到突变菌株HYT-057,利用木糖产油能力大幅度提高,油脂产量达到7.23g/L,是原始菌株的2.56倍。经过多次传代证明其遗传性状稳定。

Using Rhodosporidium toruloides HYT-05 as parent strain, a mutant yeast strain HYT-057 having high lipid yield was screened by microwave irradiation mutation, which using xylose as sole carbon. The lipid yield of HYT-057 reached 7.23 g/L, which was as 2.56 times as that of parent swain. In addition, its genetic property remained stable after cultured for many times.