肝脏肿瘤高分辨魔角旋转质子波谱分析

Metabolic Profiles in Human Hepatic Tumors Analyzed by In Vitro High-resolution Magic-angle-spinning ^1H NMR Spectroscopy

应用高分辨魔角旋转质子核磁共振波谱分析了正常肝脏(9例)、良性肿瘤(7例)及肝细胞癌(9例)代谢物变化.半定量分析表明,肝细胞癌组织的甘氨酸、牛黄酸、胆碱、谷氨酸盐、丙氨酸、缬氨酸及乳酸明显高于正常对照和良性肝脏肿瘤组织.实验结果表明肝细胞癌的代谢特征不同于正常肝脏和良性肝脏肿瘤.不同肝脏组织的代谢改变可以通过高分辨魔角旋转质子核磁共振波谱检测.

The metabolic profiles in normal human liver tissues （n= 9）, benign hepatic tumor tissues （n=7） and hepatocellular carcinoma （HCC, n=9） tissues were measured by high-resolution magic angle spinning ^1H nuclear magnetic resonance （HR-MAS ^1H NMR） spectroscopy. Semi-quantitative analyses showed that the HCC tissues contained significantly higher levels of glycine, taurine, choline, glutamate, alanine, valine and lactate, as compared to the normal and benign liver tissues. The results of this study suggest that the metabolic profiles in the HCC tissues were significantly different from normal liver tissues and benign hepatic tumor tissues, and such differences may be used as references for the diagnosis of HCC.