一株海洋锰氧化细菌对Mn(Ⅱ)的氧化去除研究

Mn(Ⅱ) Oxidation and Removing By A Manganese-Oxidizing Bacterium from Deep Sea

锰氧化微生物能够将可溶的Mn2+氧化为不溶的锰氧化物沉淀,因此在生物除锰上具有重要应用价值。以1株分离自太平洋深海的锰氧化细菌Brachybacterium sp.Mn32为试验对象,研究了其最佳生长pH值和NaCl质量体积分数,Mn2+抗性以及对Mn2+的氧化去除能力等。结果表明,Mn32在pH值7.0时生长最快(pH值4～10下生长),最佳生长NaCl质量体积分数为4.5%(0～15%下生长)。Mn2+对Mn32的最小抑菌浓度为55 mmol·L-1,在2 d之内Mn32对0.2 mmol·L-1 Mn2+的去除率达到99%以上。电镜扫描观察表明,所得到的锰氧化物存在于Mn32细胞的间隙和表面。

In this research,a Mn-oxidizing bacterium Brachybacterium sp.Mn32 from Pacific Ocean was studied on its optimal growth conditions as pH and NaCl,Mn^2＋ resistance,and abilities of Mn^2＋ oxidation and removing.The results showed that pH7.0 and 4.5% NaCl were optimal for the growth of Mn32.MIC of Mn^2＋ to Mn32 was 55 mmol·L^-1.Mn32 was able to oxidize and be removed more than 99% of 0.2 mmol·L^-1 Mn^2＋ in 2 days.Scan electron microscope observation showed that the produced Mn oxides located in the adjunct spaces and on the cell surfaces of Mn32.