摘要
采用分光光度法,以人参皂甙Rbl为对照,选择波长为571nm,建立了测定竹节人参皂甙含量的方法。实验结果表明,皂甙含量在100—600ug之间,皂甙的质量与吸光度具有明显线性关系。在此范围内,建立的皂甙质量(ug)和吸光度之间回归方程为Y=392.47x+104.18(R^2=0.9985)。检测方法的加样回收率为99.54%.RSD为0.97%。测定方法与高效液相色谱法的测定结果无显著差异。
Saponins of panax japonicus was determined by spectrophotometry at 571 nm wavelength with ginsenoside Rbl as a reference. The results showed linearity between the quantity and the absorbance, and the linear range of saponins was 100 - 600 ug. The regression equation was Y = 392.47x + 104. 18 ( R^2 =0. 998 5). The average recovery rate was 99.54% and the RSD was 0. 97%. The method is comparable to HPLC. The method is simple, rapid and accurate.
出处
《应用化学》
CAS
CSCD
北大核心
2009年第3期358-360,共3页
Chinese Journal of Applied Chemistry
基金
湖北省自然科学基金(2006ABA036)资助项目
生物资源保护与利用湖北省重点实验室开放基金项目(2007020)
关键词
竹节人参
皂甙
分光光度法
测定
panax japonicus, saponin, spectrometric method, determination