分光光度法测定竹节人参甲醇提取物中皂甙含量

Determination of Saponins in Methanol Extract from Panax Japonicus by Spectrophotometry

采用分光光度法，以人参皂甙Rbl为对照，选择波长为571nm，建立了测定竹节人参皂甙含量的方法。实验结果表明，皂甙含量在100—600ug之间，皂甙的质量与吸光度具有明显线性关系。在此范围内，建立的皂甙质量（ug）和吸光度之间回归方程为Y=392．47x＋104．18（R^2=0．9985）。检测方法的加样回收率为99．54％．RSD为0．97％。测定方法与高效液相色谱法的测定结果无显著差异。

Saponins of panax japonicus was determined by spectrophotometry at 571 nm wavelength with ginsenoside Rbl as a reference. The results showed linearity between the quantity and the absorbance, and the linear range of saponins was 100 - 600 ug. The regression equation was Y = 392.47x ＋ 104. 18 （ R^2 =0. 998 5）. The average recovery rate was 99.54% and the RSD was 0. 97%. The method is comparable to HPLC. The method is simple, rapid and accurate.