粗山羊草间杂交及抗条锈病新基因遗传分析和分子标记

Intraspecific Hybridization among Aegilops tauschii Varieties & Genetic Analysis and Molecular Markers of a Novel Stripe Rust Resistance Gene from A. tauschii

粗山羊草是小麦野生近缘属种,是D基因组的供体,蕴含大量的抗病资源,是进行小麦遗传改良的重要资源.选取条锈病免疫材料Y206和高度感病材料Y121杂交后代进行遗传分析和抗病性鉴定.从粗山羊草[Aegilops tauschii (Coss.)Schmal]Y206中鉴定出1个显性抗小麦条锈病基因,暂定名为YrY206.并利用SSR分子标记对该抗病基因标记定位,应用分离群体分组法(Bulked segregant analysis,BSA)筛选到Wmc11a、Xgwm71c、Xgwm161和xgwm183标记,与该基因之间的遗传距离分别为4.0cM、3.3cM、1.5cM和9.3cM.根据连锁标记所在小麦微卫星图谱的位置,YrY206被定位在3DS染色体上,可能是一个新的抗小麦条锈病基因.

As the wild relatives of wheat and D genome donor of wheat, Aegilops tauschii with many resistant resources, and is an important genetic resource for wheat breeding. Genetic analysis and disease resistance identification were made for the wheat stripe rust of filial generation from immune material Y206 and high sensitive Y121. A dominant wheat stripe rust resistance gene was detected from A. tauschii （Coss.） Schmal Y206. The novel gene was temporarily designated as YrY206. Using the microsatellite marker location on the chromosome, by bulk segregation analysis, four microsatellite markers Wmcl1α, Xgwm71c, Xgwm161 and Xgwm183 were found and linked to YrY206 with genetic distances of 4.0, 3.3, 1.5 and 9.3 cM, respectively. According to the locations of the linked markers, the resistance gene was located on chromosome 3DS and YrY206 may be a novel stripe rust resistance gene. Fig 2, Tab 2, Ref 22