SLE患者T淋巴细胞4—1BB的表达及其临床意义

Expression of 4-1BB on T-lymphocytes with Systemic Lupus Erythematosus and Its Clinical Sig-nificance

[目的]探讨系统性红斑狼疮（SLE）T淋巴细胞上共刺激分子4-1BB的表达，及其与临床活动性指标的关系。[方法]应用流式细胞术检测40例SLE患者和20例正常对照者外周血T细胞活化前后4-1BB的表达。[结果]SLE患者CD4^＋ T和CD8^＋T细胞表达的4-1BB明显高于正常对照组（P〈0．01），用抗CD3单抗体外刺激后CD4^＋T和CD8^＋ T细胞表达的4-1BB均显著高于活化前（表达百分率为26．13±7．25和24．12±5．47，P〈0．01）。SLE患者T淋巴细胞活化前后CD4^＋T细胞上4-1BB的表达均高于CD8^＋T细胞上4-1BB的表达（均P〈0．01）。SLE患者淋巴细胞活化前后的4-1BB^＋CD4^＋T细胞百分数均与IgG和尿微量白蛋白呈正相关关系（r=0．623，P〈0．01，r=0．407，P〈0．01，r=0．605，P〈0．01，r=0．463，P〈0．01）。[结论]SLE患者T淋巴细胞活化前后CD4^＋T和CD8^＋T表达的共刺激分子4—1BB均明显升高，4—1BB可能参与淋巴细胞的异常活化及肾脏损伤。

[Objective]To investigate the expression of costimulatory molecule 4-1BB on T-lymphocytes of patients with systemic lupus erythematosus（SLE） and its relationship with the clinical active index . [Methods] The expression of 4-1BB on T-lymphocytes from 40 SLE patients and 20 healthy controls was detected by flow cytometry. [Results]The expression of 4-1BB on CD4^＋ T and CD8^＋ T lymphocytes from SLE patients was significantly higher than that of normal controls. There was higher expression of 4-1BB on CD4^＋ T and CD8^＋ T lymphocytes stimulated with anti-CD3 antibody from SLE patients （26.13±7.25 and 24.12±5.47, P 〈0. 01） than that before activation. The expression of 4-1BB on CD4^＋ T was higher than that of CD8^＋ T before and after T cell activation （ P 〈0.01, P 〈0.01）. In addition, the expression of 4-1BB on CD4^＋ T before and after activation in SLE patients was positively correlated with the level of IgG and the amount of urinary micro-albumin. [Conclusion]The expression of 4-1BB on CD4^＋ T and CD8^＋ T lymphocytes from SLE patients before and after T cell activation increases significantly. The expression of 4-1BB on CD4^＋ T cell may take part in immune inflammation of lupus nephritis by abnormally activated CD4^＋ T celt.