α-乳香酸对HL-60细胞株VEGF分泌及其Flt-1受体表达的影响

Effect of a-boswellic Acid on VEGF and Its Receptor Expression in Acute Promyelocytic Leukeomia Cell Lines HL-60 Cells

[目的]探索不同浓度α-乳香酸作用于白血病细胞株HL-60细胞末同时间后，细胞血管内皮生长因子（VEGF）mRNA表达及VEGF蛋白分泌及受体Flt-1的变化。[方法]MTT法检测乳香酸对细胞增殖的抑制作用，选择适当的药物浓度及作用时间；RT-PCR法检测α-乳香酸处理前后HL-60细胞中VEGFmRNA表达水平；流式细胞术检测α-乳香酸处理前后细胞中VEGF和受体Flt-1蛋白表达水平。[结果]α乳香酸在15．0mg／L时在体外能下调HL-60细胞VEGFmRNA的表达和VEGF蛋白的分泌并呈时间与剂量依赖性（P〈0．05）。[结论]α-乳香酸在体外可以抑制急性早幼粒细胞白血病血管新生，其作用可能与下调VEGF及Fit-1有关，具有抗白血病效应。

[Objective] To assess the effects of α-boswellic acid on VEGF and Flt-1 expression in acute promyeiocytie leukemia cell Lines HL-60 cells. [Methods] HL-60 cells were treated with α-boswellic acid, then cell proliferation was examined by the method of MTT. The level of VEGF-mRNA expression was distin- guished by semi-quantitative RT-PCR technique. Flow cytometry was applied to examine the protein expression of VEGF and Flt-1. [Results] Within the concentration of （10.0-15.0）mg/L, α-boswellic acid strongly inhibited proliferation of HL-60 cells. When the drug was 15.0mg/L, α-boswellic acid inhibited the mRNA expression of VEGF and the protein expression of VEGF and Flt-1. [Conclusion] Within a certain drug concentration, α-boswellic acid can inhibit the proliferation of HL-60 cells. The role of inhibiting angiogenesis maybe relates with down regulating of the expression of VEGF and Fh-1.