结石性胆囊炎患者胆囊黏膜、胆汁和结石中幽门螺杆菌的检测研究

Study on detection of Helicobacter pylori in tunica mucosa vesicae felleae,bile and stone in patients with lithic cholecystitis

目的检测单纯结石性胆囊炎患者胆囊黏膜、胆汁和结石中幽门螺杆菌(H.pylori)DNA,探讨H.pylori感染与胆石形成的关系。方法选用一对来源于H.pylori特异性尿素酶A基因的引物,用PCR法对56例结石性胆囊炎患者的胆囊黏膜、胆汁和胆石中的H.pylori DNA进行检测。结果结石性胆囊炎患者胆汁中H.pylori DNA的阳性率为46%,显著高于胆囊黏膜(23%)和胆石(14%)(P均<0.05)。结论结石性胆囊炎患者胆石形成过程中H.pylori感染起着重要的作用。

Objective It is to approach the role of Helicobacter pylori in forming of gallstone by detecting the DNA of Helicobacter pylori in tunica mucosa vesieae felleae, bile and stone in patients with lithic cholecystitis. Methods The DNA of Helicobacter pylori in tunica mucosa vesicae felleae, bile and stone in 56 patients with lithic cholecystitis was detected by polymerase chain reaction （PCR） method, with a single primer pair stem from the nucleotide sequence of the specific urease Agene of Helicobacter pylori. Results The positive rate of Helicobacter pylori in bile was 46 % （ 11 cases） which was remarkably higher than that in mucus （23 % , 13 cases） and In stone （14 % , 8 cases） （P all 〈 0.05）. Conclusion The infection of Helicobacter pylori is an key factor in forming of gallstone in patients with lithic cholecystitis.