摘要
目的应用高效液相色谱法对盐杜仲进行指纹图谱的研究。方法色谱柱Agilent XDB-C18(150mm×4.6mm,5μm),乙腈-1%乙酸溶液二元梯度洗脱,体积流量1mL·min-1,检测波长277nm。结果根据选择的色谱条件制订了盐杜仲的指纹图谱分析方法。结论该方法简便可行,重复性好,色谱图对盐杜仲中各成分得到了较好的分离,可为盐杜仲的质量控制提供一定技术依据。
OBJECTIVE To study a fingerprint of Eucornmia ulrnoides Oliv. processing product by HPLC. METHODS Agilent XDB-- C18 column （ 150mm ×4.6mm, 5 μm） was used, with mixtures of acetonitrile and 1% acetic acid as mobile phase in a gradient mode. The flow rate was 1mL·min^-1 and wavelength was 277nm. RESULTS According to the optimum chromatographic conditions,good fingerprint of Eucornrnia ulmoides Oliv. processing product has been described. CONCLUSION The method is simple and accurate with good reproducibility. It may be practical value for the quality control of Eucommia ulmoides Oliv. processing product.
出处
《齐鲁药事》
2009年第2期84-85,共2页
qilu pharmaceutical affairs
关键词
杜仲
盐制品
高效液相色谱
指纹图谱
Eucommia ulmoides Oliv.
processing product
HPLC
fingerprint
