摘要
目的建立酒石酸唑吡坦口腔崩解片含量测定的反相高效液相色谱法。方法色谱柱为AlltimaC8柱(250mm×4.6mm,5μm),流动相为甲醇-0.1mol·L-1醋酸铵溶液(60∶40);流速为1.0mL·min-1;检测波长310nm。结果线性范围为20～200μg·mL-1,r=0.9999,平均回收率为99.92%,RSD=0.32%(n=15)。结论本法灵敏度高,重现性好,结果准确、可靠。
OBJECTIVE To establish a RP-HPLC method for determination of Zolpidem Tartrate Orally Disintegrating Tablets. METHODS Alltima C8 column （250mm×4. 6mm,5μm） was used as the stationary phase,methanol--0. 1mol· L^-1 ammonium acetate solution （60 ： 40）was used as the mobile phase at 1.0mL·min^-1 ;The detection wavelength was 310nm. RESULTS The standard curve was proved to be linear in the range of 20-200μg·mL^-1 , r =0. 9999. The average recovery rate was 99.92%, RSD was 0. 32 % （ n = 15）. CONCLUSION The method is sensitive,reproducible,accurate and reliable.
出处
《齐鲁药事》
2009年第2期86-87,共2页
qilu pharmaceutical affairs
