不同基质共代谢降解2,4,6-三硝基苯酚的研究

Study on co-metabolic biodegradation of 2,4,6-trinitrophenol with different substrates

以葡萄糖、可溶性淀粉、乙酸钠和苯酚作为共基质,通过35℃恒温生化培养箱缺氧驯化污泥,间歇恒温振荡试验研究了在不同共代谢基质条件下,2,4,6-三硝基苯酚(TNP)的缺氧降解性能。结果表明,经过驯化的微生物加入葡萄糖和苯酚作为碳源,在TNP∶苯酚∶葡萄糖为10∶3∶100的比例下,TNP生物降解率可以从不加碳源时的31.2%提高到86.6%;不同共基质条件下TNP的降解均符合一级反应动力学方程,反应速率常数大小依次是K苯酚<K葡萄糖<K葡萄糖+苯酚,其中葡萄糖和苯酚同时作为共基质时TNP降解速率常数最大为0.019 h-1。

The biodegradation of 2,4,6-trinitrophenol（TNP） was studied by the way of biochemical domes- tication of microorganism in an intermittent constant temperature rocking-bed with glucose, dissoluble amylum, acetic natrium and phenol as co-substrates under different co-metabolic conditions. The research confirmed that when domesticated microorganism were provided to glucose and phenol as carbon sources and kept the weight proportion of TNP： phenol： glucose being 10： 3： 100, the biodegradation rate of TNP could be enhanced from 31.2% to 86.6% ; under different co-substrate conditions, the degradation of TNP was in accordant with the first order kinetic equation. The order of the reaction rate constants is Kphenol 〈 Kglucose 〈 Kglucose＋phenol, and glucose and phenol is an outstanding co-metabolism substrate whose reaction rate constant is 0. 019 h^-1 .