微量金属元素及其配合物对厨余垃圾甲烷发酵的影响

Effect of trace metal and its complexation on methane fermentation of food residue

生物可利用的微量金属元素不仅能够保证污染物以最大的速率转化,而且还可以使某些特殊的转化得以发生,并提高微生物对有毒污染物质的耐受能力。在研究厨余垃圾总固体浓度(total solid,TS)、接种量和C/N比对厨余垃圾厌氧发酵影响的基础上,重点探讨微量金属元素钴及其配合物丝氨酸对厨余垃圾厌氧发酵甲烷产量及关键酶含量的影响。结果表明,当TS为0.5%、接种污泥量为100 mL/L和C/N比为20∶1时,厨余垃圾厌氧发酵的甲烷产率较高,为367 mL/gCOD;添加2μmol/L的微量金属元素钴-配合物丝氨酸时,甲烷产率则提高到432 mL/g COD,相应地,辅酶M的含量由空白实验的41.21μmol/g VSS提高到54.64μmol/g VSS,辅酶F420的含量由0.31μmol/g VSS提高到0.48μmol/g VSS。

Bioavailable trace metal could achieve the optimum conversion of pollutants into methane, promote the special conversion and improve the tolerance of microorganism to toxic substances. Based on the investigation of the effects of total solid, inoculum dosage and C/N ratio on anaerobic fermentation of food residue, the influence of trace metal Co and its complexation serine on the methane production and key coenzeme contents was evaluated. The results showed that the methane production achieved the maximum with 367 mL/g COD under 0.5% of TS, 100 mL/L of inoeulum dosage and 20：1 of C/N ratio. With the addition of 2 umol/L trace metal Co and its complexation B, it increased to 432 mL/g COD. The contents of coenzyme M and F420 went up to 54.64umol/g VSS and 0. 48 umol/g VSS from 42.21umol/g VSS and 0.31 umol/g VSS, respectively.