茶树轮斑病病原菌毒素生物测定方法研究

Research on Bioassary Method of Pestallozzia theae Toxin

[目的]比较不同生物测定方法在茶树轮斑病病原菌毒素生物检测上的敏感性,筛选其中较好的方法。[方法]用叶片浸渍法、针刺法、涂抹法、根伸长测定法、根冠细胞分析法等生物测定方法测试10种非寄主植物叶片和6种非寄主植物根对茶树轮斑病病原菌毒素的敏感性。[结果]茶树轮斑病病原菌毒素原液对非寄主植物的离体叶片都有致病作用,不同植物反应不同;6种生物测定方法中根冠细胞分析法效果较好。[结论]试验表明根冠细胞分析法快速、简便、精确,是一种能较好地检测茶树轮斑病病原菌毒素毒性的生物测定方法。

[Objective] The research aimed to compare the sensitivity to Pestallozzia theae toxin with ditterent bioassary methods, and to screening the best method. [Method]With the leaf impregnation method, acupuncture method, smear meanings,mot elongation measuring meanings,the cell of root cap analytic method, 10 kinds of non-host plants＇leaf blade and 6 kinds of non-host plants＇ root had been tested to check their sensitivity to P. theae toxin. [Result]The stock solution of P. theae toxin had pathopoiesis on the detached leaves of non-host plants. And different plants had different reactions to the toxin. [Conclusion]The experiment proved that, the cell of the root cap analytic method was fast,simple and precise. It could be seen as the better bioassaries to detech P. theae toxin.