利用基因扫描分析TCR Vβ亚家族的CDR3长度方法检测T细胞克隆性

T CELL CLONALITY IDETIFICATION USING GENESCAN BY CDR3 SIZE OF T CELL RECEPTOR Vβ GENE

分析了健康人、Ｔ－ＡＬＬ外周血及Ｔ细胞株Ｍｏｌｔ－４和Ｊｕｒｋａｔ中ＴＣＲＶβＴ细胞的表达和克隆性。应用ＲＴ－ＰＣＲ扩增ＴＣＲＶβ２４个亚家族的ＣＤＲ３，并经基因扫描和序列分析确定Ｔ细胞克隆性。结果表明健康人表达除Ｖβ２０外的多克隆Ｔ细胞；Ｔ－ＡＬＬ仅表达３～４个Ｖβ亚家族Ｔ细胞；部分呈寡克隆性；Ｍｏｌｔ－４和Ｊｕｒｋａｔ分别表达Ｖβ２和Ｖβ８单克隆Ｔ细胞。Ｔ－ＡＬＬ中存在克隆性生长Ｔ细胞。该方法是检测Ｔ细胞克隆性的敏感和精确的方法。

 cell clonality and T cell receptor(TCR) Vβ subfamily expression in peripheral blood of health and TALL cases or T cell line Molt4 and Jurkat cells were analyzed. The complementarity determining region 3(CDR3) of TCR 24 Vβ subfamily genes was amplificated using RTPCR, the products were further studied by genescan and sequencing analysis for identification of T cell clonality. Besides Vβ20, the expression of all subfamily TCR Vβ polyclonal T cells were found in normal cases. But only some Vβ subfamily T cells were expressed in 2 cases with TALL, and the oligoclonal T cell could found in some subfamily of TCR Vβ. Molt4 and Jurkat cells expressed only Vβ2 or Vβ8 subfamily and showed monoclone T cell in PCR products. It is a sensitive and exact method to identify T cell clonality. T cell clonal expansion may be found in TALL.