猪囊尾蚴重组体的培养及其诱导表达

Culture and Expression of Cysticercus Cellulosae Recombinant

将猪囊尾蚴λｇｔ１１重组体转染ＥｃｏｌｉＹ１０９０并经ＩＰＴＧ诱导表达融合蛋白（ＦＰ），观察重组体数量、培养时间及ＩＰＴＧ浓度对ＦＰ产量的影响，结果表明，重组噬菌体数量及培养时间对ＦＰ制备有较大影响，ＩＰＴＧ的浓度对ＦＰ制备有调节作用。单克隆ＦＰ经简化－Ｗｅｓｔｅｒｎｂｌｏｔ（ＳＷＢ）检测，λＣＣ２检出率最高，为７３．３％；４个克隆以等比联合应用（λＣＭ），其检出率为８６．７％，高于单克隆ＦＰ的检测结果，应用λＣＣ２及λＣＭ检测其特异性均为１００％。

Cysticercus Cellulosae recombinants infected E coli Y1090 and were induced to express fusion protein (FP) by IPTG,then we observed the effects of the quantity of recombinants,cultural time and the concentration of IPTG on the production of FP.The results showed that the quantity of recombinants and cultural time greatly influenced the production of FP,the concentration of IPTG played a role in adjusting the production of FP.Each monoclone FP was tested by Simplified-Western blot (SWB),the positive rate of λCC2FP was the highest,73.3%;using SWB multiclone of FP (1∶1∶1∶1) was more efficient,the positive rate was 86.7%,and both the specific rate were 100%.