期刊文献+

IL-12、IL-7、IL-2协同诱导PBMC所产生的内源性细胞因子对其细胞毒活性的影响 被引量:1

Effect of Endogenous Cytokines on Synergistic Inducement of Cytotoxic Activity of PBMC by IL12,IL7 and IL2
下载PDF
导出
摘要 应用细胞因子生物学检测法对IL-12、IL-7、IL-2协同诱导的PBMC培养上清中的TNF和IL-2进行了检测,同时观察了抗TNF-α、抗TNF-β和抗IL-2抗体对刺激细胞的细胞毒作用的影响。结果表明,IL-12、IL-7、IL-2三种因子协同刺激PBMC可产生较高水平的TNF,而抗TNF-α单抗能显著抑制刺激细胞的杀瘤活性,提示内源性TNF-α在介导该类细胞的细胞毒效应中起重要作用。 The bioassay were used to estimate the level of TNF and IL2 in the supernatant of PBMC induced by IL12,IL7 and IL2.The effect of antiTNFα,antiTNFβ and antiIL2 antibody on cytotoxicity of PBMC were detected.The results showed that synergistic stimulation of IL12,IL7 and IL2 could produce high concentration of TNF in culture supernatant,and antiTNFα could obviously inhibit the cytotoxic activity of treated PBMC,therefore,the endogenous TNFα was considered playing important role in the cytotoxic activity of treated PBMC.
出处 《哈尔滨医科大学学报》 CAS 1998年第2期81-83,共3页 Journal of Harbin Medical University
关键词 细胞因子 细胞毒 肿瘤 白细胞介素 生物疗法 Cytokine Cytotoxicity Tumor
  • 相关文献

参考文献6

  • 1Naume B,et al.A comparative study of IL-12(cytotoxic lymphocyte maturation factor),and IL-2,IL-7-induced effects on immunomagnetically purified CD56^+ NK cells.J Immunol,1992;148:2429.
  • 2Gately MK,et al.Regulation of human cytolytic lymphocyte responses by interleukin-12.Cell Immunol,1992;143:127.
  • 3吴厚生.用^3H-TdR释放法测量细胞介导的细胞毒功能[J].上海免疫学杂志,1987,(4):230-233.
  • 4Meager A,et al.Assays for tumour necrosis factor and related cytokines.J Immunol Methods,1989;116:1.
  • 5Tada H,et al.An improved colorimetric assay for interleukin 2.J Immunol Methods,1986;93:157.
  • 6Naume B,et al.Effecta of IL-7 and IL-2 on highly enriched CD56^+ natural killer cells.J Immunol,1991;147:2208.

共引文献40

同被引文献1

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部