水溶液悬浮聚合法制备妥拉苏林分子印迹聚合物微球及其性能研究

Preparation and Characteristics of Molecularly Imprinted Polymer Microspheres for Tolazoline by Aqueous Suspension Polymerization

以药物妥拉苏林为印迹分子,甲基丙烯酸（MAA）为功能单体,乙二醇二甲基丙烯酸酯（EDMA）为交联剂,采用水溶液悬浮聚合方法,制得对妥拉苏林有较好选择识别能力的球形分子印迹聚合物（molecularly imprinted polymer,MIP）。扫描电子显微镜（SEM）照片显示MIP微球粒径为50～200μm,静态平衡吸附实验表明,MIP对妥拉苏林的吸附量是95.28μmol/L,而空白分子印迹聚合物（non-imprinted polymer,NIP）的吸附量是52.66μmol/L,氮气吸附法测得MIP和NIP的比表面积分别为27.17 m^2/g和10.27 m^2/g。将所得的MIP应用于固相萃取（SPE）的固定相,结果表明,该MIP能选择性吸附妥拉苏林,当以结构类似的药物萘甲唑啉为竞争底物时,分离因子可达1.75。MIP用于人尿样的分离和富集时,回收率为99%～114%,相对标准偏差为1.5%～2.5%。

The molecularly imprinted polymeric microspheres for tolazoline were prepared by aqueous suspension polymerization, using α-methacrylic acid （MAA）as functional monomer and ethylene dimethacrylate（EDMA） as cross linker. The molecularly imprinted polymer（MIP） was characterized by scanning electron microscope （SEM）, nitrogen adsorption and static adsorption experiments. The SEM image exhibited that the sizes of mierospheres ranged from 50 to 200 μm. The specific surface areas analysis indicated that the MIP and non-imprinted polymer（NIP） had an average surface area of 27. 17 and 10. 27 m^2/g, respectively. Binding studies were performed to evaluate uptake of MIP and NIP with a result that MIP has a significantly higher binding capacity for tolazoline than NIP. The maximum static adsorption capacities of the MIP and NIP for tolazoline were 95.28 and 52. 66 μmol/ L, respectively. Using naphazoline, which has similar structure with tolazoline, as a competitive molecule, the tolazoline MIP showed a considerable selectivity to tolazoline with the separate factor up to 1.75. The MIP, as the sorbent, was applied to separate and enrich tolazoline in human urine samples with recoveries of 99% - 114% and RSDs of 1.5% - 2.5%.