姜黄素对人宫颈癌细胞Caski侵袭转移的影响

Effects of Curcumin on Invasion and Metastasis of Human Cervical Cancer Cells Caski

【目的】研究姜黄素对人宫颈癌细胞Caski侵袭转移的影响并探讨其可能的机制。【方法】以10,25,50μmol/L姜黄素处理Caski细胞24,48,72h后,MTT法检测其对Caski细胞的生长抑制作用。应用Transwell小室进行人工重组基底膜(Matrigel)侵袭和运动实验,观察姜黄素对Caski细胞侵袭和转移的影响。Westernblot检测细胞中MMP-2、MT1-MMP和NF-κB蛋白水平变化。【结果】应用姜黄素处理后人宫颈癌细胞生长受到抑制且作用呈时效-量效依赖关系;同时细胞侵袭和迁移能力明显降低;MMP-2,MT1-MMP和NF-κB蛋白的表达均明显减弱。【结论】姜黄素能够减弱人宫颈癌细胞Caski的侵袭和转移,其机制可能与降低MMP-2,MT1-MMPandNF-κB的表达有关。

[ Objective ] To explore the effects of cm＇cumin on invasion and metastasis of the human cervical cancer cells Caski and to investigate the possible mechanism. [Method] Caski cells were treated with 10, 25, and 50 μmol/L curcumin for 24, 48, and 72 h, respectively. Proliferation of Caski cells was measured with MTT assay. Invasion and metastasis of Caski cells were evaluated with Transwell chamber. The expression of MMP-2, MT1-MMP, and NF-κB were detected by Western blot. [Result] After being treated with curcumin, the proliferation of Caski cells was inhibited in a time and dose-depending manner. The invasion and metastasis of Caski cells were also inhibited. The expression level of MMP-2, MT1-MMP, and NF-κB were decreased. [Conclusion] Curcumin could affect the invasion and metastasis of the human cervical cancer cells Caski. It is probably one of its molecular mechanisms to inhibit the expression of MMP-2, MT1-MMP, and NF-κB.