神经支配对兔后肢缺血诱导侧支血管生长过程中巨噬细胞标记物、细胞间黏附分子和血管细胞黏附分子表达的影响

Involvement of innervation in the expression of ICAM,VCAM and RAM11 during collateral vessel growth in the rabbit hindlimb induced by ischemia

目的:通过比较去除支配血管壁的神经,观察神经支配对侧支血管巨噬细胞标记物(RAM11)、细胞间黏附分子(ICAM)和血管细胞黏附分子(VCAM)表达的影响。方法:20只新西兰大白兔,左侧行股动脉结扎,右侧行股动脉结扎加坐骨神经和股神经切除,7d后将动物处死,应用免疫荧光组织化学技术,观察单纯结扎和结扎加去神经侧RAM11、ICAM和VCAM在侧支血管的表达变化,兔前肢大小相似的正常血管用作正常对照。结果:正常血管ICAM和VCAM的表达非常低,外膜中可见数目很少的RAM11阳性细胞;股动脉结扎侧,侧支血管ICAM、VCAM和RAM11的表达均明显上调,RAM11阳性细胞主要在外膜聚集,在中膜也可见,ICAM表达主要在血管内皮细胞,而VCAM除了在血管内皮细胞有表达外,在外膜也有表达;股动脉结扎加去神经侧,ICAM、VCAM和RAM11的表达比股动脉结扎侧有所下调,其荧光强度的比较有统计学意义。结论:在缺血诱导的兔后肢侧支血管生长模型中,去除血管壁的神经导致了炎症分子ICAM、VCAM和RAM11的阳性细胞减少,表明血管壁神经对RAM11、ICAM和VCAM的表达调节有重要影响。

Objective： To investigate the effect of innervation on the expression of RAM11, ICAM and VCAM in the collaterl vessel growth. Methods： Twenty rabbits were operated on both left and right femoral artery iigation. On the right side, additional sciatic and femoral nerve incise were also performed. After a 7-day survival, the animals were sacrificed. The ex- pression of RAMll, VCAM and ICAM was detected by confocal immunofluorescence with special antibodies against RAM11, ICAM and VCAM. Results： In normal small arteries, the expression of ICAM was absent, and VCAM was very low and only presented in endothelial cells; In only /igated-side collateral vessels, the expression of RAMll, ICAM and VCAM was significantly increased （P〈0.05） as compared to the normal arterial vessels. RAM11 in the adventitia was evident; ICAM was mainly localized in the endothelium. Increased VCAM were found in both adventitia and endothelinm; In contrast, dennervation led to a significant reduction of RAMll, ICAM and VCAM expression （P%0.05）. Conclusion： This data suggest that the nerve of the vascular wall is involved in mediating expression of RAM11, ICAM and VCAM. It will serve as a spring-board for luther study on the role of neural regulation in inflammation formation during collateral vessel growth.