TBX3基因mRNA在乳腺癌中表达的检测及其意义

Expression of TBX3 mRNA and its role in the pathogenesis and metastasis of breast cancer

目的探讨TBX3基因表达在乳腺癌发病过程的作用。方法常规提取51例液氮保存的新鲜乳腺癌和对应的癌旁组织总RNA。将这些总RNA逆转录成cDNA后,利用实时荧光定量PCR的方法检测TBX3基因在这些组织中的表达情况,分析其与乳腺癌转移相关的临床病理参数之间的关系。结果与癌旁乳腺组织相比,TBX3基因在乳腺癌组织中呈明显高表达。此外,在有转移的癌组织中,TBX3基因表达比非转移的癌组织明显增强。结论TBX3基因高表达提示其促进了乳腺癌的发病过程,并可能与乳腺癌转移发生相关。

Objective To explore the role of TBX3 gene in the pathogenesis of breast cancer. Methods The total RNA of 51 fresh breast cancer tissues and the corresponding adjacent tissues were extracted and reverse transcribed into cDNA to detect the expression of TBX3 mRNA by real-time PCR. The correlation between TBX3 mRNA expression and the clinicopathologic parameters in relation to breast cancer metastasis was analyzed. Results Compared to that in the adjacent tissues, the expression of TBX3 mRNA was markedly increased in breast cancer tissues. TBX3 mRNA expression was significantly higher in metastatic breast cancer than in non-metastatic tumors. Conclusion Increased expression of TBX3 mRNA suggests the involvement of TBX3 in the pathogenesis and metastasis of breast cancer.