强脉冲光对大鼠皮肤转换生长因子-β1mRNA表达的影响

Effect of intense pulsed light on transforming growth factor-β1 mRNA expression in rat skin

目的观察强脉冲光(IPL)对大鼠皮肤转换生长因子-β1(TGF-β1)mRNA表达的影响,探讨光子嫩肤的分子生物学机制。方法选择15只SD大鼠,每只选3个部位用强脉冲光照射,采用同一照射参数,能量密度34J/cm2,分为3个脉冲,脉宽各为4、5、6ms,脉冲延时为20ms及25ms。照射后第1、3、5、7、15、30天分别于治疗及非治疗部位切取皮肤样本,原位杂交检测,观察。结果照射部位第1天皮肤表皮、真皮细胞均呈阳性染色,第7天达高峰,第15天染色渐弱,第30天只有真皮细胞呈弱阳性染色,非治疗部位染色阴性。结论强脉冲光照射皮肤后可引起TGF-β1mRNA表达的增加,提示TGF-β1在光子嫩肤过程中对皮肤的重塑起重要作用。

Objective To observe the effect of intense pulsed light （IPL） on transforming growth factor-β1 mRNA （TGF-β1 mRNA） expression in rat skin and explore the molecular mechanisms ofphotorejuvenation. Methods Fifteen SD rats were exposed to IPL in 3 dermal regions with triple pulses （duration of 4, 5, and 6 ms） at the energy density of 34 J/cm^2 and pulse delay of 20 or 25 ms. On days 1, 3, 5, 7, 15, and 30 after the treatment, skin specimens from the treated and non-treated areas were obtained to detect TGF-β1 mRNA expression with in situ hybridization. Results In the UPL-exposed skin areas, TGF-β1 mRNA expression was detected in the epidermal keratinocytes and dermal cells 1 day after the exposure, reaching the highest expression level on day 7 followed by gradual decrement since day 15, and till day 30, only weak expression was found in the dermal cells. In the non-exposed regions, the cells remained negative for TGF-β1 mRNA. Conclusion IPL can enhance TGF-β1 mRNA expression in the skin, suggesting that TGF-β1 plays an important role in dermal remodeling in photorejuvenation.