卵泡液中蛋白氧化水平与IVF-ET结局参数的关系

Relationship between protein oxidation levels in the follicular fluid and the outcome parameters of in vitro fertilization-embryo transplantation

目的探讨体外受精-胚胎移植(IVF-ET)周期卵泡液中蛋白氧化水平与IVF-ET结局参数的关系。方法测定64例因卵管性因素不孕接受IVF-ET治疗的女性患者卵泡液中晚期氧化蛋白产物(AOPP)水平,研究AOPP水平与IVF-ET结局参数的关系。结果(1)卵泡液中AOPP水平与成熟卵母细胞比例(r=-0.401,P=0.001)、受精率(r=-0.257,P=0.045)、卵裂率(r=-0.290,P=0.024)、良好胚胎形成率(r=-0.520,P=0.000)均呈显著负相关;(2)不同获卵数组之间AOPP水平有显著性差异(F=3.851,P=0.027),其中以获卵数8～15个组AOPP水平最低,获卵数<8个组最高;(3)非妊娠组AOPP水平显著高于妊娠组(t=3.665,P=0.001);(4)不同年龄组AOPP水平差异有显著性意义(F=15.919,P=0.000),其中年龄>35岁组AOPP水平最高,<30岁组最低。结论IVF-ET周期中,卵泡液中存在蛋白氧化应激现象。卵泡液中高水平的AOPP可能对卵母细胞和早期胚胎发育造成不良影响,继而影响IVF结局。

Objective To explore the relationship between protein oxidation levels in the follicular fluid and the outcome parameters of in vitro fertilization-embryo transplantation （IVF-ET）. Methods The levels of advanced oxidation protein products （AOPP） in the follicular fluid were measured in 64 women with tubal infertility undergoing IVF-ET. The relationship between the AOPP levels and IVF-ET outcome parameters was analyzed. Results AOPP levels showed significant inverse correlations between the proportion of mature oocytes （r=-0.401, P=0.001）, fertilization rate （r=-0.257,P=0.045）, cleavage rate （r=-0.290, P=0.024） and good embryo rate （r=-0.520, P=0.000）. AOPP levels differed significantly between the grrups with different retrieved oocyte numbers （F=3.851, P=0.027）, being the lowest in women with 8 to 15 retrieved oocytes and the highest in those with retrieved oocytes below 8. The AOPP level in the non-pregnant women was significantly higher than that in the pregnant women （t=3.665, P=0.001）. The AOPP levels also differed significantly with age （F=1 5,919, P=0.000）, and the women 〉35 years of age had the highest level and those below 30 years had the lowest level. Conclusion Protein oxidative stress is present in the follicular fluid of women on IVF-ET cycles. High level of AOPP may have adverse effects on the oocytes and early embryonic development and may affect the outcome of IVF-ET.