白介素-17A在哮喘小鼠中的表达及意义

Expression of interleukin-17A in asthmatic mice and its significance

目的初步探讨哮喘小鼠中血清白介素(IL)-17A的水平及在肺、脾及胸腺组织中的表达情况。方法28只BALB/c雌性SPF级小鼠随机分为正常对照组(A组)和哮喘模型组(B组),各14只;观察两组小鼠气道病理改变及BALF中细胞比例变化;采用双抗体夹心酶联免疫吸附试验(ELISA)方法检测两组小鼠血清IL-17A水平及肺、脾和胸腺组织匀浆预孵后的表达情况。结果B组小鼠气道炎症以嗜酸性粒细胞及嗜中性粒细胞浸润为主,而A组无此变化。B组外周血及肺、脾、胸腺组织匀浆中IL-17A的水平显著增高,与A组比较差异有显著性(P<0.01)。B组肺组织中IL-17A水平与BALF中嗜中性粒细胞(r=0.693,P=0.040)和嗜酸性粒细胞呈正相关(r=0.733,P=0.030)。结论IL-17A在哮喘小鼠血清及肺、脾及胸腺组织中高表达,IL-17A可能是参与支气管哮喘急性发作的主要细胞因子之一,在哮喘中的作用可能与其促进中性粒细胞及嗜酸性粒细胞在气道内聚集有关。

Objective To study the serum level of interleukin- 17A （IL- 17A） and its expressions in the lung, spleen and thymus in asthmatic mice. Methods In 14 normal BALB/c female mice and 14 asthmatic mice, the changes in the airway pathology and the cell proportion in the bronchoalveolar lavage fluid （BALF） were observed. The serum level of IL-17A and IL-17A expressions in the tissue homogenates of the lung, spleen and thymus of the mice were detected by sandwich enzyme-linked immunosorbent assay （ELISA）. Results The airway inflammation in the asthmatic mice was characterized mainly by eosinophil and neutrophil infiltration, which was not observed in the normal control group. Serum IL-17A levels and IL-17A expressions in the lung, spleen and thymus of the asthmatic mice were significantly higher than those in the normal control group （P〈0.01）. In the asthmatic mice, IL-17A expression in the lung tissues was positively correlated with the percentages of neutrophils （r=0.693, P=0.040） and eosinophils （r=0.733,P=0.030） in the BALF. Conclusions IL-17A is highly expressed in the serum, lung, spleen and thymus of asthmatic mice. IL-17A may be one of the major cytokines involved in exacerbation of bronchial asthma, and is probably associated with the recruitment of neutrophils and eosinophils into the airways.