电刺激小脑顶核对抑郁症大鼠额叶单胺类递质含量的影响

Effect of electrical stimulation of cerebellar fastigial nucleus on the contents of monoaminergic transmitters in frontal lobe of rats with depression

目的观察电刺激小脑顶核（FN）对抑郁大鼠额叶单胺类递质含量的影响，探讨电刺激小脑顶核治疗抑郁症的途径及机制。方法选择健康成年雌性Wistar大鼠30只，随机分为正常对照组、刺激对照组、模型组、模型假刺激组、齿状核（DN）刺激组（DN组）及顶核刺激组（FN组），每组5只。正常对照组不造模，除进行麻醉外，不给予任何处理；刺激对照组不造模，接受手术操作和电刺激FN；模型组为模型动物；模型假刺激组为模型动物，电极安置部位为FN，但不接受电刺激（仅留置针电极1h）；DN组为模型动物，给予电刺激DN1h；FN组为模型动物，给予电刺激FN1h。建立Wistar大鼠抑郁症模型后，于模型大鼠左侧小脑顶核或齿状核放置电极并进行电刺激；应用荧光分光光度法分别测定大鼠两侧额叶脑组织内5-羟色胺（5-HT）、去甲肾上腺素（NE）和多巴胺（DA）含量。结果与正常对照组相比，模型组大鼠双侧额叶内5-HT及NE含量显著下降（均P〈0．01），而DA含量无明显改变。刺激对照组动物接受FN刺激后，5-HT、NE和DA水平均无明显改变（均P〉0．05）。FN组动物接受FN刺激后，左侧和右侧额叶NE及5-HT含量均有显著增高（均P〈0．05），右侧增高更明显，双侧额叶NE及5-HT含量相比，差异有统计学意义（均P〈0．05）。DN组动物接受DN刺激后，左侧和右侧额叶5-HT、NE和DA水平均无明显变化（均P〉0．05）。结论电刺激FN可显著增加抑郁大鼠额叶内NE及5-HT含量，提示其对抑郁症可能具有治疗作用。

Objective To investigate the effect of electrical stimulation of cerebellar fastigial nucleus （FN） on the contents of monoaminergic transmitters in frontal lobes of rats with depression, and to explore the pathway and mechanism of electrical stimulation of cerebellum in treating depression. Methods A total of 30 Wistar rats were used to establish the animal depression model by exposure to chronic stressing circumstances. Electrical stimulation delivered to the left cerebellar FN or left dentate nucleus （DN） for 1 h was used as the intervention. The contents of serotonin （5-HT） , noradrenalin （NE） and dopamine （DA） in frontal lobe were determined with fluorospectrophotometry. Results Compared with normal control group, the contents of 5-HT and NE in bilateral frontal lobes of depression model rats decreased significantly （P 〈 0. 001 ）. No significant change of all three monoaminergic transmitters in normal rats was observed after stimulation of FN or DN （ P 〉 0.05 ） , but significant increases of 5-HT and NE in right and left frontal lobes were observed in depression model rats after stimulation of FN （ P 〈 0.05, P 〈 0. 001; P 〈 0.05, P 〈 0.01 ） , with the increase in right lobe more significant than in left lobes （P 〈 0.05 ）. In depression model rats underwent DN stimulation, significant increase of contents of all three monoaminergic transmitters in frontal lobes was observed. Conclusion Electrical stimulation of cerebellar FN can increase the contents of 5-HT and NE in frontal lobes of rats with depression, and this suggest that electrical stimulation might have therapeutic effect to depression.