母源性BDE-209暴露的子鼠脑组织发育及突触超微结构及数量的观察

Effect of moternal BDE-209 exposure on neural development and the ultramicrostructure of synapsis of offsprings

目的:观察暴露于十溴联苯醚(brominated diphenyl ethers-209,BDE-209)的子鼠脑组织发育的情况,电镜下观察子鼠突触超微结构及数量的变化。方法:经胃灌法建立母源性BDE-209动物模型(300mg/kg.d-1,以精制花生油溶解)。实验组(A):自购买日始母鼠胃灌BDE-209直至生育第一代子鼠断乳;实验组(B):自购买日始胃灌单纯花生油至妊娠,妊娠后改为胃灌BDE-209直至生育第一代子鼠断乳;实验组(C):自购买日始胃灌单纯花生油至分娩新一代子鼠,仅在哺乳期胃灌BDE-209;对照组(D):自购买日始一直胃灌单纯等量花生油。每组选取子代鼠作为研究对象,制作组织标本及电镜标本;尼氏染色光镜下观察子鼠脑组织的形态学变化;透射电镜下观察子鼠脑组织的结构及突触结构和数量的变化。结果:(1)经尼氏染色,子鼠成年后的脑组织形态学变化不明显,未发现特殊结构的差异;(2)电镜下见实验组子鼠脑细胞及突触超微结构发生变化;(3)突触密度计数:各组大鼠子代海马CA3区分子层内突触密度计数(个/70μm2)结果显示,在母鼠接触BDE-209的过程中,A组与B、C、D组比较,差异均有显著统计学意义(P<0.05)。B、C组与对照组比较无显著差异,B组与C组之间差异无统计学意义(P>0.05)。结论:母鼠接触BDE-209可以导致子鼠脑神经细胞及突触超微结构发生改变,影响子鼠突触的形成发育,降低突触数量。

groups and the control group by transmission electron microscope. （2）The density of synapsis suggested the group A had much sparse than the control group and the other experlmantal groups. The Objective：To study the effect of moternal BDE-209 exposure on offsprings＇ neural development and the uhramicrostructure and density of synapsis. Methods：Peanut oil suspensions of commercial deca-BDE was given in doses of 300mg/（ kg · d） by oral gavage for the experimental groups, including Group A （ the group exposed to deca-BDE all life long）, Group B（the group exposed to deca-BDE 0nly in gestation and lactation）, Group C （only exposed to deca-BDE in lactation）. The control group （Group D） was administered only with the same capacity of peanut oil at the same time. All of the specimens come from the experimental animals based on the groupA, B, C, D. The nerve cell was observed by Nissl＇s staining using light microscope. The uhramicrostructure and density of synapsis was observed by transmission electron microscope. Results：There were no obvious histomorphology changes between the experimantal groups and the control group by Nissl＇s staining using light microscope. （ 1 ） There were some ultramicrostructure changes of synapsis could be observed between the experimantal difference had statistical significance. But not so as to the others. Conclusion：Long term moternal BDE-209 exposure can affect the the development of nerve cell and change the ultramicrostructure of synapsis and lower the density of synapsis.