HER-2在宫颈病变脱落细胞的表达及临床意义

Expression and clinical significance of HER-2 oncogene in cervical lesion cells

目的:研究癌基因HER-2在不同程度宫颈病变脱落细胞的表达,探讨它在宫颈癌发生发展中的作用及临床意义。方法:用荧光原位杂交(FISH)法检测12例正常宫颈、55例宫颈上皮内瘤变(CIN)及24例浸润性宫颈癌脱落细胞中HER-2的表达。结果:HER-2在正常宫颈脱落细胞中不表达,在CINⅠ、CINⅡ～Ⅲ及宫颈癌的阳性表达率分别为6.3%、25.6%及58.3%。HER-2在CINⅡ～Ⅲ及子宫颈癌组表达均显著高于正常组(P<0.01),同时宫颈癌组HER-2明显高于CIN组(P<0.01)。HER-2高表达与细胞分级明显相关(P<0.01),与临床分期、病理类型无关。结论:HER-2基因高表达在宫颈癌发生发展中可能起重要作用,在宫颈病变脱落细胞中检测HER-2基因对探讨宫颈病变的进展和预测不良预后可能有重要意义。

Objective：To investigate the expression of the HER-2 oncogene in cervical lesion, and to evaluate the clinical significance of HER-2 expression in cervical lesion. Method：The expression of HER-2 in 24 cases with infiltrating cervical cancer,55 cases with cervical intraepithelial neoplasia （CIN） and 12 cases with normal control cervix were detected by the fluorescent in situ hybridization （FISH）. Results：The expression of HER-2 was not observed in normal cervix cells. Positive expression rates of the HER-2 in CIN Ⅰ , CIN Ⅱ - Ⅲ and infiltrating cervical cancer cells were 6.3% ,25.6% and 58.3% respectively. Compared with control group, HER-2 expression rate in CIN Ⅱ - Ⅲ and infiltrating cervical cancer was different significantly （P 〈0.01 ） ,and there was a significantly difference between CIN group and infiltrating cervical cancer group（P 〈0.01 ）. The over expression of HER-2 was correlated with the differentiation degree of carcinoma closely （ P 〈 0.01 ）. No correlation was found between positive expression and clinical stages, nor pathologic types. Conclusion：The over-expression of HER-2 may play an important role in cervical carcinogenesis, it may be recommended to evaluate the progression and predict poor prognosis of cervical lesion.