摘要
目的观察H102对APP转基因小鼠脑内胆碱能系统及自由基的影响。方法将APP转基因鼠(n=16)随机分为模型组和给药组,每组8只。并设同月龄同背景C57BL/6J小鼠为正常对照组。对照组、模型组每日侧脑室注射生理盐水,给药组每日侧脑室注射H102(80μmol·L^(-1))生理盐水溶液。采用免疫组化和生化方法测定小鼠脑组织海马及皮质内ChAT、AcHE、SOD的活性及:MDA的含量。结果(1)ChAT免疫组化染色:H102给药组皮质及海马CA3区ChAT阳性神经细胞排列较规则,胞浆ChAT阳性染色较明显,可见阳性染色神经纤维,较密集。治疗组海马CA3区及颞叶皮质ChAT阳性细胞数及阳性率明显高于模型组(P<0.01)。(2)H102给药组海马AcHE活性及皮质MDA含量明显低于模型组(P<0.01或0.05),而皮质SOD活性明显高于模型组(P<0.01)。结论H102可明显改善提高ChAT、SOD的活性,降低AcHE活性,降低MDA的含量,这可能是其改善痴呆认知和记忆障碍的作用机制。
AIM To observe the effects of H102 on cholinergic system and free radicals of APP transgenic mouse. METHODS The APP transgenic mouse were radomly divided into model group and H102 treatment group, and a group of C57BL/6J mice with the same age and background was set as normal control group. Model group and the normal control group were treated with saline solution, while the H102 treatment group was treated with compounds （80 μmol·L^-1 H102 in saline solution）, medicine or saline was infused in lateral cerebral ventricle every day. Immunohistochemical stain was done with ChAT and biochemistry method was used to determine the activities of ACHE, SOD, and the content of MDA. RESULTS （1） The ChAT staining results showed that there were generous and orderly ChAT positive neuron in hippocampus CA3 domain and cortex of temporal lobe of mouse in H102 treatment group, the ChAT positive staining in plasma was darker, and there were dense positive fibres. The number and rate of ChAT positive neuron in H102 treatment group were obviously higher than the model group （P 〈 0.01 ）. （2） The activities of AchE and content of MDA in H102 group were obviously lower than the model group （P 〈 0.01 or 0.05）, while the activity of SOD was higher （P 〈 0.01）. CONCLUSION H102 can obviously improve the activities of ChAT and SOD, lower the activity of ACHE, and decrease the content of MDA, which might be the mechanism of H102 to improve the cognitive impairment and dysmnesia of Alzheimer＇s disease.
出处
《中国新药与临床杂志》
CAS
CSCD
北大核心
2009年第2期106-110,共5页
Chinese Journal of New Drugs and Clinical Remedies
基金
天津市科技攻关培育项目05YFGPGX07100
