性病门诊男性就诊者尿道解脲支原体分群分型研究

Study on the subtypes of Ureaplasma urealyticum isolated from STD clinic attenders

目的:探讨性病门诊男性就诊者尿道解脲支原体(Uu)分群分型的特点。方法:采用病例对照研究,收集性病门诊单纯Uu阳性的男性NGU和非NGU就诊者尿道拭子标本,通过PCR法检测Uu基因分群和生长代谢抑制试验进行Uu血清分型。结果:男性就诊者尿道Uu基因群1占24.3%(18/74),基因群2占59.5%(44/74),基因群1,2同时阳性6.8%(5/74),Uu分布均以基因群2为主,Uu基因群1,2在男性NGU组与非NGU组间分布没有统计学差异(P均>0.05);Uu血清分型显示,NGU和非NGU组优势血清型均以2、4、7、14型为主,且在两组间差异无统计学意义(P均>0.05)。结论:不同基因群、不同血清型Uu在男性NGU患者与NGU就诊者之间的分布无差异,提示与非淋菌性尿道炎无相关性;本地区性病门诊男性尿道解脲支原体以血清型2、4、7、14型为主。

Objective：We seek to explore the relationship between U. urealyticum subtype 1 and 2 with nongonococcal uretbritis. Methods：Urethral samples were collected from patients with and without non - gonocoecal urethritis. PCR were performed to subtype U. urealyticum. Serotypes of U. urealyticum were also classified. Results： Of all the samples, 24. 3 % （ 18/74 ） were tested positive for subtype 1, 59.5% （44/74） were positive for subtype 2, 6.8% （5/74） were positive for both subtype 1 and subtype 2. Therefore, subtype 2 was dominant among samples tested. There was no statistically significant difference in percentage of subtype 1 or subtype 2 among patients with or without urethritis. The serotyping result showed that serotype 2,4,7,14 were dominant in both urethritis and non -urethritis group. Conclusion：The result from this study suggests it is unlikely that any specific subtype and serogype of U. urealyticum is related to the development of NGU. Serotypes 2,4,7,14 were dominant strains in the Guangdong region.