FQ-PCR与细胞培养检测女性HSV感染者宫颈排泌HSV的对照研究

FQ-PCR and Cell Culture Detect HSV Shedding from Cervix Asymptomatic Females Seropositive for HSVgG2-IgG

目的:比较FQ-PCR与细胞培养方法检测无症状生殖器疱疹宫颈排泌HSV的阳性率。方法:用型特异性抗体-2型单纯疱疹病毒糖蛋白G-IgG(HSVgG2-IgG)筛选出阳性女性34例,每例连续2月每月在排卵期和月经前取宫颈拭子,共取材136个。每次取材的拭子分别使用细胞培养鉴定和荧光定量多聚酶反应(Fluorescent quantitation-polymerase chain reaction,FQ-PCR)检测宫颈分泌物中HSV。结果:FQ-PCR方法得到38个拭子阳性,均为HSV-2;细胞培养方法得到4个拭子阳性,经直接免疫荧光分型鉴定为HSV-2。即所有宫颈拭子HSV阳性标本均为HSV-2,无HSV-1。采用Mc-Nemer检验细胞培养与FQ-PCR检测结果阳性率的差异有统计学意义(P<0.05),FQ-PCR检测的阳性率比细胞培养法高。结论:对于女性无症状生殖器疱疹,FQ-PCR的方法检测宫颈排泌HSV优于细胞培养方法。

Objective： We aim at comparing methods of FQ - PCR and cell culture for detection of HSV shedding from cervix of asymptomatic female objects who tested positive for HSVgG2 - IgG. Methods ：34 asymptomatic females who tested positive for HSVgG2 -IgG were selected using subtyping ELISA kits. Cervical secretion samples were collected during the ovulation and premenstrum period for consecutive 2 months. FQ - PCR was used to detect HSV - 2 DNA. Each sample was divided into two portions, to tested for cell culture and for FQ - PCR. Results： Out of 136 samples, HSV - 2 DNA was found in 38 samples by the method of FQ - PCR, whereas using cell culture we detected HSV - 2 DNA only from 4 samples. All viruses detected were HSV - 2 in typing and no HSV - 1 was found. There is statistical significance in the positive rate of samples between methods of cell culture and FQ - PCR when it is analyzed by McNemer test. A higher positive rate was found with FQ -PCR method than that with culture method. Conclusion：FQ -PCR would be the clinically preferable method to detect HSV shedding from cervix of HSVgG2 - IgG seropositive females without symptoms.