甲壳低聚糖-硒对高血糖大鼠血清抗氧化酶和胰岛细胞的改善作用

Effect of Chitooligosaccharide-Selenium on Serum Antioxidase Activity and Islet Cells in Hyperglycemic Rats

目的观察甲壳低聚糖-硒(COS-Se)对高血糖大鼠血清抗氧化酶活力及大鼠胰岛细胞的保护作用。方法制备高血糖大鼠模型,分别给予甲壳低聚糖-硒、硒、甲壳低聚糖对其进行干预,定期检测超氧化物歧化酶、谷胱甘肽过氧化物酶的活力;第20周末取胰腺组织进行切片形态学观察。结果在提高糖尿病模型大鼠SOD活力方面,COS-Se与对照组比较,具有较明显的作用(P<0.05);但COS-Se、Se以及COS效果基本一致(P>0.05);在改善糖尿病模型大鼠GSH-Px活力方面,COS-Se与对照组比较,差异有显著性(P<0.01);但COS-Se与Se两者的作用强度未见明显区别(P>0.05)。结论COS-Se对2型糖尿病大鼠的胰岛细胞具有保护作用,同时还能够改善血清抗氧化酶的活力。

Objective To investigate the effect of chitooligosaccharide-selenium （COS-Se） treatment on serum antioxidase activity and pancreatic islets in hyperglycemic rats. Methods Ten normal Wistar rats were included in the normal control group. Hyperglycemic rat model was established by both high-fat diet and following a single injection of low-dose streptozotocin. 80 hyperglycemic rats were randomized into four groups covering control, COS-Se, Se and COS groups. The serum antioxidase activity was determined at different time intervals. After 20 weeks of experiment, the pancreatic islets were examined by histopathology. Results COS-Se showed a protective effect on islet cells notably. Compared with the control group, the activity of serum SOD in the COS-Se group was increased significantly （P 〈 0.05）, but there was no significant difference among the COS-Se group, Se group and COS group （P 〉 0.05）. Compared with the control group, COS-Se significantly improved the activity of GSH-Px （P 〈 0.01）, but there was no significant difference between the COS-Se group and Se group （P 〉 0.05）. Conclusion COS-Se can protect islet ceils and improve serum antioxidase activity.