小型猪胰淀素基因的多态性分析

Polymorphism Analysis of IAPP Gene in Minipigs

目的探讨我国3个特有的小型猪品系,巴马小型猪,五指山小型猪,中国农大小型猪胰淀素（IAPP）基因多态性分布,为我国小型猪在2型糖尿病及代谢性疾病研究中的应用提供基础资料。方法提取3个品系小型猪血液基因组DNA,针对IAPP基因外显子3~内含子3的部分序列进行PCR扩增,产物鉴定、测序,统计分析基因多态。结果在所扩增的片段中共检测出2个SNPs位点,SNPs1：43G→A,位于外显子上,未引起的氨基酸的改变,突变发生在五指山猪（G/A杂合突变为16.7%,A纯合突变为83.3%）和中国农大猪（G/A杂合突变60%,A纯合突变为20%两个品系中。SNPs2：214C→T,位于内含子上,发生在五指山猪（T/C杂合突变为16.7%,T纯合突变为83.3%）和中国农大猪（T/C杂合突变为20%,T纯合突变为60%）两个品系中。结论在IAPP基因外显子3-内含子3的部分扩增序列中发现了2个SNPs位点,在3个品系小型猪中的分布不同。

Objective To analyze the single nucleotide polymorphism （SNPs） of islet amyloid polypeptide in three Chinese minipig strains, Bama minipigs, Wuzhishan minipigs and Nongda minipigs, to provide basic data for their application in studies on diabetes meltitus and metabolic diseases. Methods The genomic DNA was extracted from the three strains of native Chinese miniature pigs. A segment lAPP gene exon 3 was amplified by PCR. The amplified products were sequenced and identified, and were compared with the results of NCBI. Results Two SNPs were identified. SNPsl： 43G→A, was in exon region and no amino acid frans for motion was caused. The mutation was discovered in Wuzhishan minipigs （G/A heterozygous 16.7 %, A homozygous 83.3 % ） and Nongda minipigs （ G/A heterozygous 60 %, A homozygous 20 % ）. SNPs2 ： 214C→T, was in intron region. It was dicovered in the Wuzhishan minipigs （T/C heterozygous 16.7%, T homozygous 83.3%） and Nongda minipigs （T/Cheterozygous 20 %, T homozygous 60 % ）. Conclusion Two SNPs are discovered from exon 3 to intron 3 in IAPP gene sequence, and they show a different distribution in the three minipigs strains.