全反式维甲酸和三氧化二砷对人口腔鳞癌细胞系KB细胞周期的影响

Effects of all-trans retinoic acid and arsenic trioxide on cell cycle of oral squamous carcinoma cell line KB

目的研究全反式维甲酸(all-trans retinoic acid,ATRA)和三氧化二砷(arsenic trioxide,As2O3)诱导分化对人口腔未分化鳞癌细胞系KB细胞周期的影响及意义。方法采用流式细胞技术观察人口腔未分化鳞癌细胞系KB细胞经不同浓度ATRA和As2O3诱导分化后96h时的细胞周期的变化。结果ATRA作用于KB细胞后,加药组与对照组相比,被阻断在G1期的细胞比率轻度升高,S期比率下降,抑制率随浓度增高无明显升高的趋势。As2O3三个不同浓度作用KB细胞96h时,没有明显改变各细胞周期所占的比例。抑制率随作用浓度的增加而升高。结论ATRA和低浓度As2O3对人口腔未分化鳞癌细胞系KB细胞具有诱导分化作用,ATRA诱导KB细胞分化可能与G1、G0期阻滞有关。

Objective To investigate the change of cell cycle of oral squamous carcinoma cell line KB by employing all-trans retinoic acid （ATRA） and arsenic trioxide （As2O3） and to discuss the significance of such change. Methods The change of cell cycle of oral squamous carcinoma cell line KB when subject to all-trans retinoic acid （ATRA） and arsenic trioxide （As2O3） was detected by flowcytometry. Results The percentage of G1/G0 in KB cells after being subject to all-trans acid （ATRA） for 96 h was higher than that in control group,and the percentage of S was declined. The inhibitory rate did not increase with the increasing concentration of ATRA. The percentage of different phases in KB cells induced by As2O3 for 96 h was not changed. Conclusion All-trans retinoic acid （ATRA） and arsenic trioxide （As2O3） both could induce differentiation of oral squamous cell carcinoma cell line KB. All-trans retinoic acid-induced differentiation of KB cells may be related to G1 arrest.