镜鲤体重相关分子标记与优良子代的筛选和培育

Molecular markers associated with body weight of mirror carp and selection and raising of progenies

根据性状相关分子标记的QTL分析结果进行品种培育是分子标记用于水产育种研究的重要方向之一。利用镜鲤与体重相关的3个基因座(HLJ302,HLJ338,HLJ343)作为快速生长的标记,筛选出一批镜鲤亲本进行繁殖,对得到的子代进行基因分型,并将基因分型结果与检测的最大10尾个体与最小10尾个体进行比较。结果表明,最大10尾具有优势基因型为每尾1.7个,最小10尾具有优势基因型为每尾0.7个,生长速度快群体与生长速度慢群体在体重相关基因型的富集上呈现显著性差异。同时选出具有富集体重相关优势基因型的子代且生长速度快的群体一个,2008年生产出子二代。综合性状好生长速度更快的镜鲤新品系正在培育之中。

It is the one of main directions for aquaculture breeding using molecular markers that stocks were selected by the results of quantitative traits loci of economic traits. The main goal of our long term project is to get a good variety with most economic traits in high quality by marker-assisted selection. We have identified 6 microsatellite loci linked with body weight at high explained variation, and also found three of these 6 loci are homozygous in experimental population in our previous works. In this paper, using other three loci （HLJ302, HLJ338, HLJ343 ） which associated with body weight of mirror carp in breeding population, a group of mature mirror carp were selected and their progenies were also gotten. When the progenies grow up, the genotypes of those progenies were analyzed with these three loci. This paper compared the genotypes between 10 biggest fish and 10 smallest fish, the results showed that there are significant difference on dominant genotype linked body weight between fast-growth population and slowgrowth population. The results showed that 10 biggest fish have the mean positive genotypes 1.7 for body weight. Meanwhile, which proved these markers are effect for breeding again. A big population with these positive dominant genotypes has been selected （also selected by good phenotypes such as good color, good body figure etc）, and a big group of progenies of second generation has been produced. A new strain of fast-growing and good-quality mirror carp has been raised.