CNTF对慢性高眼压大鼠RGCs的保护作用

Protective effect of ciliary neurotrophic factor on rat retinal ganglion cells in a chronic ocular hypertension model

目的研究慢性高眼压大鼠玻璃体腔内注射睫状神经营养因子(ciliary neurotrophic factor,CNTF)对大鼠视网膜神经节细胞(retinal ganglion cells,RGCs)的保护作用。方法大鼠随机分成1、2、3、4周组,制作慢性高眼压模型,实验眼玻璃体注入CNTF0.5μg,对照眼注入5μl磷酸钠溶液,各实验点光镜观察和进行免疫组化检测,采用图像分析系统分析。结果HE染色光镜观察分析,1、2、3周实验组RGCs数明显多于对照组(P<0.05),RGCs略有肿胀,形态趋于正常,4周实验组和对照组形态差异不明显;GAP-43免疫组织化学检测,2、3、4周实验组RGCs层阳性染色与对照组相比有显著性差异(P<0.05),1周实验组RGCs阳性染色较少(P>0.05)。结论CNTF对慢性高眼压动物RGCs起到一定的保护作用并可促进RGCs再生。

Purpose To observe the protective effect of ciliary neurotrophic factor （CNTF） on the retinal ganglion cells in rats with chronic ocular hypertension by intravitreous injection. Methods Rats were randomly divided into 1 , 2, 3 and 4 week groups. A rat model of chronic ocular hypertension was establishied, in which the experimental eyes were injected with 0. 5 μg CNTF by intravitreal injections,and control eyes were injected with 5 μl sodium phosphate solution. Pathological changes of the retinal ganglion cells （RGCs） were observed by light microscopy, and determined by immunohistoehemical technique, with a computer image analysis sys- tem. Results The number of RGCs in 1, 2, 3 week experimental eyes increased significantly as compared with the controls （ P 〈 0.05 ）. The morphology of RGCs was closed to normal besides mild swelling. There was no significant difference in morphology between 4 week experimental and control eyes, but significant differences were found between 2, 3, 4 weeks experimental groups and control group in GAP-43 expression in RGCs by immunohistochemieal technique （P 〈0. 05）. However, there were less GAP-43-positire RGCs in 1 week experimental group （P 〉0. 05）. Conclusion CNTF could produce the protective effect on RGCs and promote regeneration of RGCs in the chronic ocular hypertension animal.