显微CT与组织切片技术在骨形态计量研究中的比较

Comparative study of micro-CT and histological section in bone morphometry

[目的]通过比较显微CT技术与组织切片技术在骨形态计量分析中的差异,探讨显微CT在骨形态三维结构研究方面的技术优势。[方法]分别应用显微CT和组织切片技术分析不同类型的骨组织标本,并从标本处理、表达形式与测试指标等方面比较两者的差异。[结果]显微CT在反映骨组织三维结构特征和对骨组织"量"与"质"变化的精确测量方面明显优于组织切片技术;而组织切片技术对反映标本局部的细胞形态和生长发育变化等方面则更具优势。[结论]显微CT技术在标本处理、表达形式两方面均提供了全新的测试手段,通过结合组织切片技术能够更加理想地反映骨组织形态和结构的特点。

[ Objective ] To investigate the technical advantages of micro - CT in the research of three dimensional structure of bone tissues over histological section technology. [ Method] Different kinds of bone tissue related specimen were observed by micro - CT and histological section, respectively. The diversity of the sample treatments, result expressions and test data were compared. [ Result ] Micro - CT showed great advantages in displaying three dimensional structure characteristics of bone tissues. Both quantity and quality changes of bone tissues could be precisely determined by micro - CT Which was much better than histological section technology. However, histology showed great advantages in displaying the cell figures, growth and differentiation. [ Conclusion] Micro - CT provides a novel test method for both sample treatments and result expressions. Compound with histolozical technology, micro - CT can display the appearance and structure of bone tissues more sufficiently.