鼻腔鼻窦鳞癌Skp2和E2F1的表达及临床意义

Expression of S.phase kinase associated protein 2 （Skp2） and E2F1 and its clinicopathological significance with rhinosinus squamous cell carcinoma

目的研究鼻腔鼻窦鳞状细胞癌和慢性鼻窦炎组织中Skp2和E2F1表达及其临床病理意义。方法49例鼻腔鼻窦鳞状细胞癌、28例慢性鼻窦炎组织常规制作石蜡包埋切片，Spk2和E2F1免疫组化染色。结果鼻腔鼻窦鳞状细胞癌Skp2和E2F1表达阳性率及其评分明显高于慢性鼻窦炎组织（P〈0．01）；鼻腔鼻窦中分化鳞状细胞癌、肿块最大径≤3cm、无淋巴结转移及未超出鼻腔鼻窦、侵犯周围组织的病例（临床分型T1N0M0）Skp2和E2F1表达阳性率及评分明显低于低分化鳞状细胞癌；肿块最大径〉3cm、有淋巴结转移及已超出鼻腔鼻窦侵犯周围组织的病例（临床分型T3N1M0、T3N2M0），两者差异有统计学意义（P〈0．01）；鼻腔鼻窦鳞状细胞癌组织中Skp2和E2F1表达呈正相关（r=0．623，P〈0．01）。结论skp2和E2F1表达可能是反映鼻腔鼻窦鳞状细胞癌发生、进展、生物学行为和预后的重要生物学标记物。

Objective To study the clinicopathological significance of Skp2 and E2F1 in the rhinosinus squamous cell carcinoma and chronic sinusitis. Methods Immunohistochemical method was used to detect the expression of Skp2 and E2F1 in the routinely paraffin-embedded sections of specimens from patients with rhinosinus squamous cell carcinoma （ n = 49）, chronic sinusitis （ n = 28）. Results The expressive positive rates and scores of Skp2 and E2F1 in rhinosinus squamous cell carcinoma were significantly higher than those in chronic sinusitis （ P 〈0. 01 ）. The expression positive rates and scores were significantly decreased in middle-differentiated rhinosinus squamous cell carcinoma. The maximal diameter of mass was less than 3cm, and no-metastasis of lymphnode or no-infiltration of regional rhinosinus can be found in T1N0M0. While in the low-differentiated rhinosinus squamous cell carcinoma, the maximal diameter of mass was larger than 3em, and metastasis of lymphnode or infiltration of regional rhinosinus can be found（ T3 N1 M0, T3 N2 M0 ） （ P 〈 0. 01 ）. The closely positive correlation was found between the expression of Skp2 and E2F1 in the rhinosinus squamous cell carcinoma. Conclusions Skp2 and E2F1 might be important biological markers for carcinogenesis, progression, biological behaviors and prognosis of rhinosinus squamous cell carcinoma.