摘要
目的:研究重组毒素MSH-Ang对喉癌Hep-2细胞的毒性。方法:应用纯化的MSH-Ang进行体外细胞毒性实验,观察对喉癌细胞的生长抑制作用。结果:重组蛋白对喉癌细胞的生长有明显抑制作用,随着浓度梯度的增加,其抑制作用明显增强,细胞的死亡率逐渐增大。结论:重组毒素MSH-Ang不仅对已知的高表达MSHR的肿瘤具有特异性杀伤作用,还是一种对多种常见肿瘤细胞具有杀伤功能的导向毒素。
Objective：To study the cytotoxicity of recombinate toxin MSH Ang to Hep-2. Method：The depurated MSH Ang were applied in cytotoxieity experiment, and the growth inhibiting action to laryngeal carcinoma cell Hep 2 were observed. Result： Recombination protein inhibited the growth of laryngeal carcinoma cell Hep-2, and its inhibiting action enhanced and corpuscular mortality rate increased along with the concentration increasing. Conclusion：Recombinant toxin MSH Ang can not only take special effect in tumors with high MSHR, but also target to many other popular tumors.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2009年第5期225-226,共2页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
重组毒素
促黑激素
血管生成素
基因工程
recombinant
toxin
melanocyte stimulating hormone
angiogenin
gene engineering
