摘要
目的体外环境下,针对人肝癌细胞生长激素受体(GHR)的不同表达状态,研究重组人生长激素(rhGH)对其胞膜表面GHR密度变化的影响。方法采用免疫细胞化学方法分别半定量测定人肝癌细胞株HepG2、SMMC7721、和QGY7701的GHR表达情况,采取50ng/mLrhGH,100ng/mLrhGH,200ng/mLrhGH干预和未处理4种干预方式体外培养上述3种细胞,然后利用放射性受体分析方法定量检测胞膜表面GHR密度变化。结果免疫细胞化学检测细胞株HepG2呈GHR高表达状态,50ng/mLrhGH、100ng/mLrhGH、200ng/mLrhGH干预后胞膜表面GHR位点数分别为8.4350±0.2396、9.3957±0.5143、8.3297±0.3133(10^3/cell),较空白对照组7.5137±0.5352(10^3/cell)明显增加(P〈0.05);细胞株SMMC7721呈GHR弱表达状态,50ng/mLrhGH、100ng/mLrhGH、200ng/mLrhGH干预后胞膜表面GHR位点数分别为3.8343±0.2590、3.8213±0.2276、3.6947±0.2722(10^3/cell),与空白对照组3.7190±0.2824(10^3/cell)差异无统计学意义(P〉0.05);细胞株QGY7701未表达GHR,各种rhGH干预后均未出现GHR表达的现象。结论rhGH明显促GHR高表达细胞株HepG2胞膜表面GHR密度增加,对GHR低表达细胞株SMMC772胞膜表面GHR密度无影响。rhGH不会改变细胞株QGY7701的GHR不表达状态。
Objective Purpose To investigate the effects of recombinant human growth hormone (rhGH) on human liver cancer expressed diffferent growth hormone receptor(GHR) in vitro. Methods The expression of GHR in human liver cancer cell lines was detected by immunohistochemistry and radioligand assays. Four groups were assigned according to the cell line treatment modes: untreated group, 50 ng/mL rhGH treated group,100 ng/mL rhGH treated group,200ng/mL rhGH treated group. Results In HepG2 which GHR was high-expressed: compared with the untreated group in which the GHR site was 7. 513 7 ±0. 535 2( 10^3/cell), expressions of GHR were significantly increased in the treated groups : the sites of 50ng/mL rhGH treated group, 100 ng/mL rhGH treated group,200 ng/mL rhGH treated group were 8.435 0 ±0. 239 6,9. 395 7 ±0. 514 3, 8. 329 7 ± 0.313 3 (10^3/cell) ( P 〈 0.05 ). SMMC7721 in which GHR was low-expressed: compared with the untreated group of which the GHR site was 3.719 0 ± 0. 282 4 ( 10^3/cell), there was no remarkable effect on the treated groups:the sites of 50 ng/mL rhGH treated group, 100 ng/mL rhGH treated group, 200 ng/mL rhGH treated group were 3. 834 3 ±0. 259 0、3. 821 3±0. 227 6,3. 694 7 ±0. 272 2 (10^3/cell) (P 〉0.05). QGY7701 always had no expression of GHR no matter it was treated with different concentrations. Conclusions rhGH can stimulate the expression of GHR in which GHR was high-expressed such as HepG2. However, such effect was not remarkable for tumor cells in which GHR was low-expressed or no-expressed in vitro.
出处
《中国肿瘤外科杂志》
CAS
2009年第1期44-47,共4页
Chinese Journal of Surgical Oncology
基金
南京市医学科技发展项目计划专项基金资助项目(YKK07097)