温度和金属离子对干扰素诱导剂-聚肌胞配对的影响

Effects of temperature and metallic cations on the combination of interferon inducer polyinosinic-polycytidylic acid (polyI:C)

为提高聚肌胞(Polyinosinic-polycytidylic acid,polyI:C)的配对效果,本文研究了温度和金属离子对聚肌胞配对的影响。实验结果显示,在45℃条件下合成的聚肌胞减色效应最强,说明在该温度下聚肌苷酸(Polyinosinic acid,PI)和聚胞苷酸(Polycytidylic acid,PC)配对最紧密;Na+、Ca2+、Mg2+、Mn2+和Zn2+能够增强聚肌胞双链结构的紧密性,当Na+浓度增加到0.085mol/L后,聚肌胞的配对接近饱和;Ca2+、Mg2+、Mn2+和Zn2+对聚肌胞结构的保护效果基本一致,而Cu2+对聚肌胞结构具有明显破坏作用。建议采用45℃合成聚肌胞并添加适当浓度的Na+和Ca2+等增强其结构的紧密性,以避免机体内高活性RNA酶的降解而失去药效。

Effects of temperature and metallic cations on the synthesis of polyinosinicpolycytidylic acid were tested to improve its structure. The results showed significant hypochromic effects of polyI.C formed at 45 ℃, which indicated that the best combination of polyI.C were achieved at this temperature, while the metallic cations including Na^＋, Ca^2＋, Mg^2＋, Mn^2＋ and Zn^2＋ could enhance the polyI：C combination. 0. 085mol/L PolyI.C combination achieved saturation when Na^＋ concentration was, and the divalent cations including Ca^2＋、 Mg^2＋、 Mn^2＋ and Zn^2＋ played positive effect on the combination. However, Cu^2＋ tend to destroy the doublestranded structure. It is suggested that polyI：C should be made at 45℃ and suitable concentration of Na^＋ and Ca^2＋ should be added to make its structure more tight in order to avoid RNase＇ s digesting. This result is useful for synthesizing polyI：C at a high quality.